Protein Info for PFR28_04071 in Pseudomonas sp. RS175

Annotation: Multifunctional CCA protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 PF01743: PolyA_pol" amino acids 4 to 122 (119 residues), 61.5 bits, see alignment E=1.6e-20 PF12627: PolyA_pol_RNAbd" amino acids 149 to 212 (64 residues), 41 bits, see alignment E=2e-14 PF01966: HD" amino acids 230 to 309 (80 residues), 33 bits, see alignment E=9.7e-12

Best Hits

Swiss-Prot: 91% identical to CCA_PSEPF: Multifunctional CCA protein (cca) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: None (inferred from 95% identity to pba:PSEBR_a5145)

MetaCyc: 58% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (408 amino acids)

>PFR28_04071 Multifunctional CCA protein (Pseudomonas sp. RS175)
MQIYKVGGAVRDRLLGIPVTDIDRVVVGATAEEMLAKGFRPVGADFPVFLDPKTGEEYAL
ARTERKNGRGYGGFVFHASPDVTLEQDLIRRDLTINAMAEDDNGHVFDPYHGQRDLEARV
LRHVSPAFAEDPLRVLRVARFAARYAPLGFKVADETLELMRQLSESGELEALTAERSWKE
ISRALMEHQPQVFIQVLRDCTALRVLMPEVDALFGVPQPEAHHPEIDTGAHTLSVLEQAA
RHRQPLTVRWACLLHDLGKGLTPEHEWPRHIAHEHKGLKPIKALNERFKAPRDCQELALL
VGQYHTHGHRALELKASTLLELLQSFDVYRRPQRFEEFIAACEMDARGRKGLEDRSYPQA
DYLRGAAAAARGVAVQPLLEKGFKGPELGEAIKRERLKALKAYKEAAS