Protein Info for PFR28_03961 in Pseudomonas sp. RS175

Annotation: dTDP-4-dehydrorhamnose reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 1 to 286 (286 residues), 265.5 bits, E=5.2e-83
• PF01370: Epimerase
  • amino acids 35 to 113 (79 residues), 21.8 bits, E=1.2e-08

Best Hits

KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 98% identity to pba:PSEBR_a5052)

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (6/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.133

Use Curated BLAST to search for 1.1.1.133

Protein Sequence (289 amino acids)

>PFR28_03961 dTDP-4-dehydrorhamnose reductase (Pseudomonas sp. RS175)
MLLGGGNALGQALIRLGAEEDIGFLAPRPPEDGWDAASLTQLLDDTRPDAVINLAYYFDW
FQAEAVSEQRLASQERAVERLAELCQHHNIILLQPSSYRVFDGSRATAYSEKDEPVPLGL
RGQALWRIEQSVRATCPQHVLLRFGWLLDDSPDGILGRFLARAEHPEELLLADDRRGNPT
PVDDAARVIISVLKQLDCAAPLWGTYHYAGHEATTPLALGQAILTEARALHPLAIESPTP
QAHAARPDAAEEPQHAVLACKKILHTFGIKPRAWRAALPSLLDRFYRHG