Protein Info for PFR28_03897 in Pseudomonas sp. RS175

Annotation: Type I restriction enzyme EcoKI M protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12161: HsdM_N
  • amino acids 7 to 113 (107 residues), 33.2 bits, E=1.2e-11
• PF02384: N6_Mtase
  • amino acids 124 to 411 (288 residues), 232.4 bits, E=1.2e-72
• PF01170: UPF0020
  • amino acids 159 to 318 (160 residues), 23 bits, E=8.8e-09

Best Hits

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 91% identity to psa:PST_0633)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Protein Sequence (513 amino acids)

>PFR28_03897 Type I restriction enzyme EcoKI M protein (Pseudomonas sp. RS175)
MTNSDIVQKLWNLCDVLRDDGINYSDYVTELVLLLFIKMEFEQVQNNDSFTHKLPEGARW
PDLASKSGLNLLDHYRQMLLDLGKNSDPLIAAIYADAQTRLKEPRHLEQLIKSLDGIDWF
SARRDGLGDLYEGLLEKNASETKSGAGQYFTPRPLIDSIIRCIKPQPGETIQDPAAGTAG
FLIAADAYIKGNTDDHYDLDEKARSFQRNRAFIGIELVPGTRRLALMNTLLHGMEGDEEG
VVHLGNALGQTGANLPKVDVILSNPPFGTAKGGGGPTRDDLTYKTSNKQLAFLQHIYRGL
KPGGRAAVVLPDNVLFEAGVGTDVRRDLMDKCNLHTILRLPIGIFYAPGVKTNVLFFQKG
TADNPRQDQGCTQRTWIYDLRSNMPRFGKRTPFGAQHLKSFEDSYGEDANGNGARAENVE
GIGVISRFRVFSREQVKERGDSLDISWIKDANSLDALDLPAPEVLAGEAMAELTETLHVL
EELMKALGADDEVAAQKLLLAEVMGLSANEDKA