Protein Info for PFR28_03835 in Pseudomonas sp. RS175

Annotation: Ferrous-iron efflux pump FieF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 transmembrane" amino acids 16 to 35 (20 residues), see Phobius details amino acids 42 to 65 (24 residues), see Phobius details amino acids 84 to 104 (21 residues), see Phobius details amino acids 116 to 136 (21 residues), see Phobius details amino acids 160 to 177 (18 residues), see Phobius details amino acids 183 to 200 (18 residues), see Phobius details TIGR01297: cation diffusion facilitator family transporter" amino acids 15 to 289 (275 residues), 198.2 bits, see alignment E=8.6e-63 PF01545: Cation_efflux" amino acids 17 to 208 (192 residues), 136.1 bits, see alignment E=1.4e-43 PF16916: ZT_dimer" amino acids 212 to 288 (77 residues), 79.1 bits, see alignment E=2.2e-26

Best Hits

Swiss-Prot: 47% identical to FIEF_YERE8: Cation-efflux pump FieF (fieF) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: None (inferred from 95% identity to pba:PSEBR_a4931)

MetaCyc: 46% identical to Zn2+/Fe2+/Cd2+ exporter (Escherichia coli K-12 substr. MG1655)
RXN0-6; TRANS-RXN0-200; TRANS-RXN0-244

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (297 amino acids)

>PFR28_03835 Ferrous-iron efflux pump FieF (Pseudomonas sp. RS175)
MTTSTEHARLLRLATRASVAVALILIVAKALAWWLSGSVSMLAGLTDSALDGVTSLLNLL
AVHYALRPADDDHRYGHGKAESLAGMAQALFIGGSAVLIALQAFERMKAPVPVDAPWLSV
GVIVFSLVLTLALLALQHRVIRATGSNAVRADSLHYRSDLLLNGSILVALVLAGLGWFQV
DAWFGLGIAAYILWSAVQIARESFAVLMDEELPPDVSQHMLELACAVPGVLGAHDLRTRI
SGNHWFVQLHLELPGDLTLSVAHGLSDQAADAIHQAYPKAEVLVHADPSEVVKHTSA