Protein Info for PFR28_03672 in Pseudomonas sp. RS175

Annotation: Glutathione hydrolase proenzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 614 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR00066: gamma-glutamyltransferase" amino acids 62 to 606 (545 residues), 372.5 bits, see alignment E=1.5e-115 PF01019: G_glu_transpept" amino acids 75 to 609 (535 residues), 498 bits, see alignment E=1.9e-153

Best Hits

KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 92% identity to pba:PSEBR_a4774)

Predicted SEED Role

"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.2.2

Use Curated BLAST to search for 2.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (614 amino acids)

>PFR28_03672 Glutathione hydrolase proenzyme (Pseudomonas sp. RS175)
MLTALKPNLHRLSAVSLLAVALTLAACKAPPSSTAPPALPTAPEIASGYRTDLQTRHADK
HMAAAANPLAAEAGREMLRRGGSAIDAAIAMQAVLTLVEPQSSGIGGGAFIVLWDGKAVR
TYDGRETAPAGATEKLFLQADGKPMPFTAAQIGGRSVGTPGVLRALELAHRKHGRLKWAS
LFEPAIRLAEQGFAISPRLHSMIAADPSLPGSPDMAAYFLNADGRPKAVGTLLKNPALAG
VLKRIANEGADALYEGAIAKEIVAKVQGHANPGSLSLNDLKGYAARERAPLCTDYKRWQV
CGMAPPSSGGIAVAQILGTLQALEQRDSHAAIAPLKPLKTDKPAGLEPAPQAVHLIAEAQ
RLAYADRAQYVADSDFIPVPVKGLVDPGYLASRASLIGPRSMGVAKPGTPPGVQVAYAPD
RSPLRISTSQVVAVDDQGGAVSMTTTVESAFGSHLMVQGFMLNNQMTDFSFIPEEQGRKV
ANRVEPGKRPRSSMAPTLIFDRQSGELLAAVGSPGGSQIIEYVAKSVIGLLDWQLDPQTA
INLPNFGSRNGPTELEQGQFSPTLIQALKDKGHTVTEIDMTSGTQAIVRLRDPQGRTSLA
GGADPRREGEALGD