Protein Info for PFR28_03573 in Pseudomonas sp. RS175

Annotation: Toluene efflux pump outer membrane protein TtgI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 505 transmembrane" amino acids 14 to 32 (19 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 18 to 478 (461 residues), 367.7 bits, see alignment E=4.6e-114 PF02321: OEP" amino acids 73 to 265 (193 residues), 78 bits, see alignment E=4.2e-26 amino acids 291 to 476 (186 residues), 74 bits, see alignment E=7.1e-25

Best Hits

KEGG orthology group: None (inferred from 89% identity to pba:PSEBR_a4607)

Predicted SEED Role

"Outer membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (505 amino acids)

>PFR28_03573 Toluene efflux pump outer membrane protein TtgI (Pseudomonas sp. RS175)
MQVPRCIRRELKTLSVWVLASVIGGCIGTGGIAPQGEALPANGLATDAAIREAARDAHWP
TRQWWKAYGDRQLDRWVDLALQGSPSLAMAVARVRQARALAGVAEAAESPQINGQANLKR
HDWPTDQFYGPGELANTTTWDNNAGLGLSYALDLWGRERNASEQAVDLAHMSVAQARQAQ
LELQNNVVHAYIQLSLHYAQRDIVAATLHQQEQILELARKRLDGGIGTHFEVSQAQAPLP
ETHRQLDALDEAIALSRNQLAALAGKGPGEGARLQRPTLALGAPLKLPSALPAELLGQRP
DVVAGRWQVAAQARGIDVARAGFYPNVDLVGSLGYMATGGGALEFLTGKKLTYSVGPALS
LPIFDGGRLCAQLGEAAAGYDIAVAHYNQTLVNALKGLSDQLIRRESMDKQQAFAAESVA
AAQRTYDIALVAFQRGLTDYLSVLNAQSLLFRQQQIQQQVEAARLSAHADLVTALGGGLE
AGDDTPAAAATRPTPAPAILGALDR