Protein Info for PFR28_03528 in Pseudomonas sp. RS175

Annotation: Cytosol aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 PF02789: Peptidase_M17_N" amino acids 19 to 147 (129 residues), 112.3 bits, see alignment E=1.5e-36 PF00883: Peptidase_M17" amino acids 184 to 486 (303 residues), 437.9 bits, see alignment E=2.1e-135

Best Hits

Swiss-Prot: 92% identical to AMPA_PSEPF: Probable cytosol aminopeptidase (pepA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 96% identity to pba:PSEBR_a4559)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (496 amino acids)

>PFR28_03528 Cytosol aminopeptidase (Pseudomonas sp. RS175)
MELVVKSVSPETLKTATLVVAVGEERKLGVVATQLDALCGGAISAVLKRGDLAGKVGQSL
LLHNLPNLKADRVLLVGVGKDAELGDRPFRKIIAGVLGTLKGLGGGDAALALDELVLKGR
DSYGKNRLLVETLVDGEYQFDRFKSQKAEPRALKKVTLLTIKAAQAEVQRAVTHATAIAN
GMALTRDLGNLPPNICHPTFLGEQAKALGKAFKGLKVEVFDEKKIKDLGMGSFYSVGQGS
AQPPRLIVMQYNGGKKSEKPYVLVGKGITFDTGGISLKPGAGMDEMKYDMGGAASVFGTL
RAVLELQLPINVVGILACAENMPSGTASRPGDIVTTMSGQTVEILNTDAEGRLVLCDALT
YSERFKPQAVIDIATLTGACVVALGAHTSGLLGNNDELIGQLLSAGQQADDRAWQLPLFD
EYQEQLDSPFADIANIGGPKAGTITAACFLSRFTKNLNWAHLDIAGTAWTSGGKDKGATG
RPVPLLTQYLLDRAKA