Protein Info for PFR28_03519 in Pseudomonas sp. RS175

Annotation: Membrane-bound lytic murein transglycosylase F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 PF00497: SBP_bac_3" amino acids 21 to 244 (224 residues), 115.3 bits, see alignment E=3.1e-37 PF01464: SLT" amino acids 271 to 378 (108 residues), 87.6 bits, see alignment E=4.6e-29

Best Hits

Swiss-Prot: 94% identical to MLTF_PSEPF: Membrane-bound lytic murein transglycosylase F (mltF) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a4550)

Predicted SEED Role

"Transglycosylase, Slt family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (464 amino acids)

>PFR28_03519 Membrane-bound lytic murein transglycosylase F (Pseudomonas sp. RS175)
MLSGCVEKPNTLERVKEDGVLRVVTRNSPATYFQDRNGETGFEYELVKRFADDLGVELKI
ETADNLDDLFSQIGKPNGPVLAAAGLVSSDQRKKQVRFSHSYLEVTPQVIYRNGQSRPTD
AAALAGKKIMVLKGSTHAEQLAQLKQQYPGIEYEESDAVEVVDLLRMVDEGQIDLTLVDS
NEVAMNQVYFPNVRVAFDLGDARSQSWAVAPGEDNSLLNEINGYLDKVQKNGTLQRLKDR
YYGHVDVLGYMGATTFAQHLQQRLPKYEQHFKTYAKKEKIDWRLLAAIGYQESLWQPAVT
SKTGVRGLMMLTQNTAQAMGVSNRLDPKQSIMGGAKYLAYMKEQLDESIQEPDRTWFALA
AYNVGSGHLDDARKLAAKEGLNPNKWLDVKKILPRLSQKQWYSKTRYGYARGGEPVHFVA
NIRRYYDILTWVTQPQLEGNQVAEGNLHVPGVDKSKPNQESPQL