Protein Info for PFR28_03383 in Pseudomonas sp. RS175

Annotation: Glycine dehydrogenase (decarboxylating)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 949 transmembrane" amino acids 744 to 760 (17 residues), see Phobius details TIGR00461: glycine dehydrogenase" amino acids 14 to 942 (929 residues), 1506.1 bits, see alignment E=0 PF02347: GDC-P" amino acids 14 to 438 (425 residues), 602.2 bits, see alignment E=5.7e-185 amino acids 450 to 731 (282 residues), 41.5 bits, see alignment E=8.1e-15 PF21478: GcvP2_C" amino acids 771 to 892 (122 residues), 206.8 bits, see alignment E=7.5e-66

Best Hits

Swiss-Prot: 94% identical to GCSP1_PSEPF: Glycine dehydrogenase (decarboxylating) 1 (gcvP1) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 62% identity to ajs:Ajs_1936)

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (949 amino acids)

>PFR28_03383 Glycine dehydrogenase (decarboxylating) (Pseudomonas sp. RS175)
MTIHLGTANEFIARHIGPRASDEKAMLERLGYDSLEALSASVIPESIKGTSVLDMGDGQS
EAEALASIKAIAGKNQLFKTYIGQGYYNCHTPAPILRNLLENPAWYTAYTPYQPEISQGR
LEALLNFQTLISDLTGLPIANASLLDEATAAAEAMTFCKRLSKNKGSNAFFASRHCHPQT
LDVLRTRAEPLGIDVVVGDEHELTDVSPFFGALLQYPASNGDLFDYRELTERFHAANALV
AVAADLLALTLLAAPGEFGADVAIGSAQRFGVPLGFGGPHAAYFSTKDAFKRDMPGRLVG
VSVDRFGKPALRLAMQTREQHIRREKATSNICTAQVLLANIASMYAVYHGPKGLTQIANR
IHHLTAILAKGLGALGLSVEQESFFDTLTLHTGAQTAALHDKARAQRINLRVVDAQRLGL
SLDETTSQADVEALWSLLADGKALPDFAALAATVQSRIPAELVRQSAILSHPVFNRYHSE
TELMRYLRKLADKDLALDRTMIPLGSCTMKLNAASEMIPVTWAEFGALHPFAPAEQSAGY
QQLTSELEAMLCAATGYDAVSLQPNAGSQGEYAGLLAIRAYHQSRGEERRDICLIPSSAH
GTNPATANMAGMRVVVTACDARGNVDIEDLRAKAIEHREHLAALMITYPSTHGVFEEGIR
EICGIIHDNGGQVYIDGANMNAMVGLCAPGKFGGDVSHLNLHKTFCIPHGGGGPGVGPIG
VKSHLAPFLPGHANMERKEGAVCAAPFGSASILPITWMYIRMMGGAGLKRASQLAILNAN
YIARRLEEHYPVLYSGSNGLVAHECILDLRPLKDSSGISVDDVAKRLIDFGFHAPTMSFP
VAGTLMIEPTESESKEELDRFCDAMICIREEIRAVENGSLDKDDNPLKNAPHTAAELVGE
WTHPYSREQAVYPVASLIEGKYWPPVGRVDNVFGDRNLVCACPSIESYA