Protein Info for PFR28_03334 in Pseudomonas sp. RS175

Annotation: Adaptive-response sensory-kinase SasA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details amino acids 131 to 155 (25 residues), see Phobius details PF00672: HAMP" amino acids 153 to 207 (55 residues), 28.9 bits, see alignment 1.8e-10 PF00512: HisKA" amino acids 213 to 275 (63 residues), 52.1 bits, see alignment E=8.5e-18 PF02518: HATPase_c" amino acids 323 to 422 (100 residues), 67.5 bits, see alignment E=2.1e-22

Best Hits

KEGG orthology group: None (inferred from 94% identity to pba:PSEBR_a4371)

Predicted SEED Role

"two-component system sensor protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (425 amino acids)

>PFR28_03334 Adaptive-response sensory-kinase SasA (Pseudomonas sp. RS175)
MEFKQSLAQRIIIAFALMSALVAGAFAMGIVATVHLVEEKLISAGLGGDLQRLLLMDSVS
DWSHRPEPDQLFYFSGGPGDFELPKDLRHLERGFHEVFREQLSYHAMVEIVDGRHYVLLQ
DQSDFEERERVLFAVVLVGFVLSLALAVFLGWVLARRVMAPVVRLAQQVRHRDQLLGLAP
PLAPDYAADEVGELAVAFDATLGRLRQALTRERLFTSDVSHELRTPLMVLATSCELLLEN
PALDQRGRTQVERINRASEEMRELVQTFLMLARTQREDSGMSPRMTLGQVADNLIGLWRT
PIESKGLELIFEPGHPPDTLYNATFLTAVMGNLLRNALHYTDQGFIRLTLNSSGFVVEDS
GVGIPEEKREAMFEPFVRGNEKRGEGLGLGLSLVQRICENQGWTVSLSTMEPNGCHFEVD
LNPQP