Protein Info for PFR28_03303 in Pseudomonas sp. RS175
Annotation: Glycerophosphodiester phosphodiesterase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01126, glycerophosphoryl diester phosphodiesterase [EC: 3.1.4.46] (inferred from 93% identity to pba:PSEBR_a4344)Predicted SEED Role
"Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization (EC 3.1.4.46)
MetaCyc Pathways
- glycerol and glycerophosphodiester degradation (4/4 steps found)
- glycerophosphodiester degradation (2/2 steps found)
- phosphatidylcholine resynthesis via glycerophosphocholine (1/2 steps found)
- phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)
- anandamide biosynthesis I (3/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.1.4.46
Use Curated BLAST to search for 3.1.4.46
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (375 amino acids)
>PFR28_03303 Glycerophosphodiester phosphodiesterase (Pseudomonas sp. RS175) MPVTFTRSALMLSLLLGFGQTHAAEASTPKALATLQGIPHPAVIAHRGASFDAPESTAAA YKLARDLGADYLELDLQRSKDGVLFVLHDDSLLRTTDVATRFPERKDSPANAFTMAELKT LDAGSWFNAAYPDRARPAFAGLKILTLDEVINIAEGNPAQKPGLYIETKEPKLFPGIERD LKDKLQDRGWLSPAGSKLAKSATGVGQGKGKVVLQTFEKSSLELLHKEMPKVPKVLLLWV GEGNIEPKSKVTFAESGETDKAVYYARQEPKDRAEFEKWVRYAKDQGAIGTGPSAALTHG GSQSYADLVQPWMNQYTHDQGLLVHVYTVDEAVDFKKVMDAGVDGIFTNRASELLKYFKR PETGSVVQLLEKNGY