Protein Info for PFR28_03281 in Pseudomonas sp. RS175

Annotation: Arginine N-succinyltransferase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 341 PF04958: AstA" amino acids 1 to 332 (332 residues), 440.2 bits, see alignment E=1.9e-136 TIGR03243: arginine and ornithine succinyltransferase subunits" amino acids 2 to 334 (333 residues), 475 bits, see alignment E=1.1e-146 TIGR03244: arginine N-succinyltransferase" amino acids 2 to 334 (333 residues), 515.6 bits, see alignment E=4.8e-159

Best Hits

Swiss-Prot: 86% identical to ASTG_PSEAE: Arginine N-succinyltransferase subunit beta (aruG) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00673, arginine N-succinyltransferase [EC: 2.3.1.109] (inferred from 97% identity to pba:PSEBR_a4317)

MetaCyc: 86% identical to arginine succinyltransferase beta subunit (Pseudomonas aeruginosa)
Arginine N-succinyltransferase. [EC: 2.3.1.109]; 2.3.1.109 [EC: 2.3.1.109]

Predicted SEED Role

"Arginine N-succinyltransferase (EC 2.3.1.109)" in subsystem Arginine and Ornithine Degradation (EC 2.3.1.109)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.109

Use Curated BLAST to search for 2.3.1.109

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (341 amino acids)

>PFR28_03281 Arginine N-succinyltransferase subunit beta (Pseudomonas sp. RS175)
MIVRPVRSSDLPALIALARSTGTGLTTLPANEERLAHRVGWAEKTFRGEAGRGDADYLFV
LEDDDGRVVGISAIAGAVGLREPWYNFRVGLTVSASQELNIYREIPTLFLANDLTGNSEL
CSLFLHADYRNGLNGRMLAKARLLFIAEFPKLFGNKIIAEMRGMSDETGRSPFWESLGRH
FFKMEFSQADYLTGVGNKAFIAELMPKFPLYTCFLSEDARAVIGQVHPDTEPALSMLKSE
GFSYQGYVDIFDAGPAVECETGKIRAIRESQALVLAIGTPGDDATPFLIHNRKCLDCRIT
AAPARFASGTLVVDPLTAKRLQLNAGDQVRAVALSAARESK