Protein Info for PFR28_03277 in Pseudomonas sp. RS175

Annotation: Succinylglutamate desuccinylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03242: succinylglutamate desuccinylase
  • amino acids 6 to 327 (322 residues), 389.7 bits, E=5.6e-121
• PF04952: AstE_AspA
  • amino acids 49 to 328 (280 residues), 275.2 bits, E=3.2e-86

Best Hits

Swiss-Prot: 91% identical to ASTE_PSEPF: Succinylglutamate desuccinylase (astE) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K05526, succinylglutamate desuccinylase [EC: 3.5.1.96] (inferred from 97% identity to pba:PSEBR_a4313)

Predicted SEED Role

"Succinylglutamate desuccinylase (EC 3.5.1.96)" in subsystem Arginine and Ornithine Degradation (EC 3.5.1.96)

MetaCyc Pathways

• L-arginine degradation II (AST pathway) (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.96

Protein Sequence (335 amino acids)

>PFR28_03277 Succinylglutamate desuccinylase (Pseudomonas sp. RS175)
MLALGKLLELTLAGREPAEKTQLTVEGVRMRWLSEGALEVKPPEARDNGLDLLLSAGIHG
NETAPIELLDRLLHDIARGDIKPRARILFLFGNPEAIRRGERFVEQDVNRLFNGRHELSG
GPEALRACELERLAASFFSRPERARLHYDLHTAIRGSKIEQFALYPWKEGRQHSRRELAR
LRAAGMEAVLLQNKPSIVFSAYTYDQLGAESFTLELGKARPFGQNEGVDVSLLEIRLKQI
IEGNEPELDEGLDGLQLFSVAREVIKHSDSFRLNLPADIENFSELEKGYVLAEDIAQTRW
VIEEEGARIIFPNPKVKNGLRAGILIVPATDENLI