Protein Info for PFR28_03255 in Pseudomonas sp. RS175

Annotation: Chemotaxis protein methyltransferase Cher2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 275 PF03705: CheR_N" amino acids 7 to 58 (52 residues), 48.9 bits, see alignment 6.3e-17 PF01739: CheR" amino acids 73 to 267 (195 residues), 202.5 bits, see alignment E=7.2e-64 PF08241: Methyltransf_11" amino acids 195 to 248 (54 residues), 22.1 bits, see alignment E=3e-08

Best Hits

Swiss-Prot: 89% identical to CHER2_PSEPK: Chemotaxis protein methyltransferase Cher2 (cheR2) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00575, chemotaxis protein methyltransferase CheR [EC: 2.1.1.80] (inferred from 97% identity to pba:PSEBR_a4288)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80

Use Curated BLAST to search for 2.1.1.80

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (275 amino acids)

>PFR28_03255 Chemotaxis protein methyltransferase Cher2 (Pseudomonas sp. RS175)
MSTGNLDFEQFRVFLEKACGILLGENKQYLVSSRLNKLMEQQGLKSLGELVQRIQTQPRS
GLREMVVDAMTTNETLWFRDTYPFEVLKSKVLPAAIKASPGQRLRIWSAACSSGQEPYSI
SMSIDEFERTNIGQLKGGVQIVATDLSGTMLNNCKTGEYDSLAIGRGLSPDRLQRYFDTK
GPGRWVVKAPIKNRVEFRSFNLLDSYASLGKFDVVFCRNVLIYFSAEVKKDILLRIHGTL
KPGGYLFLGASEALNGLPDHYQMVQCSPGIIYQAK