Protein Info for PFR28_03249 in Pseudomonas sp. RS175

Annotation: Transcriptional regulatory protein RstA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 264 PF00072: Response_reg" amino acids 32 to 141 (110 residues), 92.9 bits, see alignment E=1.5e-30 PF00486: Trans_reg_C" amino acids 179 to 254 (76 residues), 85.4 bits, see alignment E=2.3e-28

Best Hits

Swiss-Prot: 38% identical to MPRA_MYCUA: Response regulator MprA (mprA) from Mycobacterium ulcerans (strain Agy99)

KEGG orthology group: K07661, two-component system, OmpR family, response regulator RstA (inferred from 93% identity to pba:PSEBR_a4282)

Predicted SEED Role

"Transcriptional regulatory protein RstA" in subsystem Orphan regulatory proteins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (264 amino acids)

>PFR28_03249 Transcriptional regulatory protein RstA (Pseudomonas sp. RS175)
MPCGKRCRHASVHIPSYSLQDEAPVEQEAWQVLIVEDDQRLAELTRDYLESNGLRVAIEG
DGAVAARRIIAEQPDLVILDLMLPGEDGLSICRKVREQYDGPILMLTARTDDTDQIQGLD
LGADDYVCKPVRPRVLLARIQALLRRSEPVEPNPQKSRRLQFGPLVVDSALREAWLQGSG
IELTSAEFDLLWLLVSNAGRILSREEIFTALRGIGYDGQDRSIDVRISRIRPKIGDDPEH
PRLIKTIRSKGYLFVPEACVDPAP