Protein Info for PFR28_03111 in Pseudomonas sp. RS175

Annotation: Hydroxypyruvate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03234: hydroxypyruvate isomerase
  • amino acids 2 to 255 (254 residues), 406.2 bits, E=2.4e-126
• PF01261: AP_endonuc_2
  • amino acids 21 to 256 (236 residues), 182.8 bits, E=4.8e-58

Best Hits

Swiss-Prot: 52% identical to OTNI_CUPNH: 2-oxo-tetronate isomerase (otnI) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K01816, hydroxypyruvate isomerase [EC: 5.3.1.22] (inferred from 95% identity to pba:PSEBR_a4157)

MetaCyc: 46% identical to 2-dehydrotetronate isomerase (Haemophilus influenzae Rd KW20)
RXN-18592 [EC: 5.3.1.35]; 5.3.1.35 [EC: 5.3.1.35]

Predicted SEED Role

"Hydroxypyruvate isomerase (EC 5.3.1.22)" (EC 5.3.1.22)

MetaCyc Pathways

• D-erythronate degradation II (1/4 steps found)
• L-threonate degradation (1/4 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.22

Use Curated BLAST to search for 5.3.1.22 or 5.3.1.35

Protein Sequence (260 amino acids)

>PFR28_03111 Hydroxypyruvate isomerase (Pseudomonas sp. RS175)
MPRFAANLSMLFTEQDFLARFEAAAKAGFSGVEYLFPYDYSSAELKALLDANGLTQVLFN
LPAGDWAKGERGIACLPDRVEEFRAGVDLAIAYAQVLGNTQVNCLAGIHPHKCDEALAEK
TFVANLRYAAEKLQAKGIKLVMEAINTRDIPGFYLNNTAQARSIREQVGSANLFLQYDIY
HMQIMEGDLARTLSAHLGEINHVQLADNPGRNEPGTGEINYRFLFEHLDRIGYQGWVGCE
YKPLTSTEAGLGWLKSHNAI