Protein Info for PFR28_03086 in Pseudomonas sp. RS175

Annotation: Chaperone protein HtpG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 634 PF13589: HATPase_c_3" amino acids 32 to 156 (125 residues), 38.7 bits, see alignment E=1.5e-13 PF02518: HATPase_c" amino acids 32 to 184 (153 residues), 28.7 bits, see alignment E=2.3e-10 PF00183: HSP90" amino acids 224 to 629 (406 residues), 434.4 bits, see alignment E=1.2e-133

Best Hits

Swiss-Prot: 95% identical to HTPG_PSEF5: Chaperone protein HtpG (htpG) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K04079, molecular chaperone HtpG (inferred from 99% identity to pba:PSEBR_a4129)

MetaCyc: 60% identical to chaperone protein HtpG (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein HtpG" in subsystem Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (634 amino acids)

>PFR28_03086 Chaperone protein HtpG (Pseudomonas sp. RS175)
MSVETQKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASDAVDKLRFEALSKPELL
EGGAELKIRVSFDKDAKTITLEDNGIGMSREEVITHLGTIAKSGTADFMKNLSGDQKKDS
HLIGQFGVGFYSAFIVADHVEVFSRRAGLAASEGVHWSSRGEGEFEVATLDKADRGTRIV
LHLKSGEDEFADGWRLRNIIKKYSDHIALPIELPKEVTAAEGEEKPEVQWETVNRASALW
TRPRTEVKDEEYQEFYKHIAHDYENPLSWSHNKVEGKLEYNSLLYVPARAPFDLYQREAP
RGLKLYVQRVFVMDQAESFLPLYLRFIKGVVDSNDLSLNVSREILQKDPIIDSMKSALTK
RVLDMLEKLAKNEPEQYKSFWKNFGQVMKEGPAEDFANKEKIAGLLRFASTQGDDGEQVV
GLADYLARAKEGQDKIYYLTGETYAQVKNSPHLEVFRKKGIEVLLLTDRIDEWLMSYLNE
FDGKSFVDVARGDLDLGNLDSEEDKKAAEEIAKSKEGLVERIKTALGDAVSEVRVSHRLT
DSPAILAIGEQDLGLQMRQILEASGQKVPDSKPIFEFNPSHPLVEKLDNEQSEERFGDLS
HILFDQAALAAGDSLKDPAAYVRRLNKLLVELSA