Protein Info for PFR28_03016 in Pseudomonas sp. RS175

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 transmembrane" amino acids 43 to 64 (22 residues), see Phobius details PF01343: Peptidase_S49" amino acids 144 to 283 (140 residues), 111.9 bits, see alignment E=1.4e-36

Best Hits

KEGG orthology group: K04773, protease IV [EC: 3.4.21.-] (inferred from 86% identity to pba:PSEBR_a4056)

Predicted SEED Role

"Periplasmic serine proteases (ClpP class)"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (329 amino acids)

>PFR28_03016 hypothetical protein (Pseudomonas sp. RS175)
MTDEWKAPAKASAENGDDKSWKLLEKTLLAGVQEQRRARRWGIFFKFLVFIYLFVGLGVI
IQAIDMKKSPGSASAYTAVIDVEGMIADKEAASADNIIGSLRAAFEDEKVKGVVLRINSP
GGSPVQSGYVYDEIVRLRAAHPDTKVYAVIADLGASGAYYIASAADQIYADKASLVGSIG
VTAAGYGFVETLDKLGIERRVYTAGEHKSFLDPFQPQKPEEIAFWQGVLDTTHKQFIASV
KKGRGDRLKDKDHPELFSGLVWSGEQALSLGLIDGLGSAGSVARDVIGEKELVDFTVEES
PLDRFSKKLGASIAERLAMWMGFQGPVLR