Protein Info for PFR28_02985 in Pseudomonas sp. RS175

Annotation: D-galactonate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02746: MR_MLE_N
  • amino acids 15 to 107 (93 residues), 93.5 bits, E=1.1e-30
• PF13378: MR_MLE_C
  • amino acids 128 to 355 (228 residues), 239.7 bits, E=3.1e-75

Best Hits

Swiss-Prot: 64% identical to DGOD_RALPJ: D-galactonate dehydratase (dgoD) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K01684, galactonate dehydratase [EC: 4.2.1.6] (inferred from 99% identity to pfo:Pfl01_4123)

MetaCyc: 62% identical to D-galactonate dehydratase (Escherichia coli K-12 substr. MG1655)
Galactonate dehydratase. [EC: 4.2.1.140, 4.2.1.6]

Predicted SEED Role

"Galactonate dehydratase (EC 4.2.1.6)" in subsystem D-galactonate catabolism (EC 4.2.1.6)

MetaCyc Pathways

• D-galactonate degradation (3/3 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)

KEGG Metabolic Maps

• Galactose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.140 or 4.2.1.6

Protein Sequence (382 amino acids)

>PFR28_02985 D-galactonate dehydratase (Pseudomonas sp. RS175)
MKITKLTTFIVPPRWCFLKVETDEGVTGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNI
EDIWTVLYRGGFYRGGAVHMSALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWI
GGDRPADTARAAKEAVGRGFTAVKMNGTEELQFLDTFEKVDLALANVAAVRDAVGPNVGI
GVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALKELAPLTSTPIALGERLFSR
WDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLD
AACYNAFIQEQSLGIHYNESNDLLDYIKDPRVFDYEKGFVKIPNGPGLGIEINEEYVIER
AAIGHRWRNPIWRHADGSFAEW