Protein Info for PFR28_02878 in Pseudomonas sp. RS175

Annotation: Dimodular nonribosomal peptide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1385 PF00668: Condensation" amino acids 31 to 478 (448 residues), 356.1 bits, see alignment E=6.9e-110 PF00501: AMP-binding" amino acids 501 to 856 (356 residues), 317.4 bits, see alignment E=3e-98 TIGR01733: amino acid adenylation domain" amino acids 521 to 931 (411 residues), 451.1 bits, see alignment E=1.7e-139 PF13193: AMP-binding_C" amino acids 915 to 990 (76 residues), 35.2 bits, see alignment 4.8e-12 PF00550: PP-binding" amino acids 1017 to 1080 (64 residues), 53.9 bits, see alignment 4.4e-18 PF00975: Thioesterase" amino acids 1104 to 1216 (113 residues), 95.6 bits, see alignment E=1.3e-30

Best Hits

KEGG orthology group: None (inferred from 84% identity to pba:PSEBR_a3916)

Predicted SEED Role

"Non-ribosomal peptide synthetase modules, pyoverdine??" in subsystem Siderophore Pyoverdine

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1385 amino acids)

>PFR28_02878 Dimodular nonribosomal peptide synthase (Pseudomonas sp. RS175)
MNELLDDDLLTLLLEEAEGSMPDGPGRDRLDRAPLSFAQQRLWLEQQRDPTRSAYNLPRA
LRLTGELDADALKQALNRVIDRHDILRTAITEIDGAAAQVVERNARLVLQRDDLGALDHQ
ARTIELARRIEQHARRPFDLSQAPLMRATLLRLDSDEHVLLLNMHHIVSDAWSNTILMQD
LTQAYARALLGDASPLPALPLQYADYAAHQRGEYLQSTACQRSGDYWRQCLGHDLPVLEL
PQDFARAASQQHMAGRVQVTLAPALAQALETFCRRQGLTPFVVALGAWQLLLGRYANQND
FTVGVPNANRNSPESQELVGFFVSSQVYRAQLDSTRTALDFLRDLRVQSLAALEHADYPL
ELILDQLQRPGAQGSNPLFQVLFNWRTGAPTTSMSIQDGLALEFLPTGDSQAKFDLSLDV
DYSPQQLLATFEYSRDLYRTATIERMAEHWQNLLQGLIDTPQCALGELQLLSSRERQQTL
DDWNRQPTQLPRERCLHHVIESHAATTGDAIALTFEGQHLSYADLNRQANRLAHRLIAEG
VGPDVLVGIAAQRTPQMIIGLLAILKAGGAYVPLDPAYPADRLAYMIQDSGITRVLTQAH
LRDSLALPAGTVCLSLDPQDVDDGCPEHNPDVPVHPDNLAYVIYTSGSTGKPKGALLAHH
NVTRLFQATDRWFSFDHRDVWCLFHSYAFDFSVWEIFGALLHGGRLVIVPHVVSRSPEDF
HALLCHERVTVLNQTPSAFKSLSQVACASRQPLDQHLRQVIFGGEAIDVQSLRPWFERFG
DRTPQLINMYGITETTVHVTWRPLSLQDLDSEAASPIGEPIVDLSWYLLDAHLNPVPKGC
TGELYVGRAGLARGYHRRADLTASRFIPDPFDPLPGGRLYRTGDLARYRADGSIEYIGRL
DHQVKIRGFRIELGEIQARLQGLPPVQDCVVLTHDGPGGLQLVAYVIATPSSGNELRESL
LAELKAHLPDYMVPSHLLFIDRFPLNANGKLDRKALPEPDASLLHARYVAPRTQLEQALA
QLWQQSLRVERVGIDDSFFELGGHSILAIELIASLKARLDITVRLQELLSNPTIAELALF
ISQKHREQAQCLVTLNQAPASCPQLFCLHPSGGIVFCYQPLARKLQNRARTFGVMHRGFS
EPHVGPEAWDEMIADYSREIVKAQPQGPYYLMGWSLGGTIALDIAATLESQGHTVGFLGL
VDSTIPEHLYPETLPRRRHVPGQEKADLASQDLVEAIEYFDLLFPTLTERTAAYRREHPE
STVKAFHDWAASQIEPGRSDLLSVVQSVKEEVMNAQAFSVHDRLLEAFDGFTFKPVRVRP
SCWWTQTEKTAQELAFCETLIQAHSVTGELQCSVHSPLRHRSMIFDDGLLDSLVEAFLGS
AAQTQ