Protein Info for PFR28_02865 in Pseudomonas sp. RS175

Annotation: Sensor histidine kinase RcsC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1092 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 445 to 463 (19 residues), see Phobius details amino acids 539 to 560 (22 residues), see Phobius details PF00497: SBP_bac_3" amino acids 63 to 274 (212 residues), 32.9 bits, see alignment E=1.2e-11 amino acids 303 to 522 (220 residues), 54 bits, see alignment E=3.9e-18 PF00512: HisKA" amino acids 580 to 646 (67 residues), 58.1 bits, see alignment 1.9e-19 PF02518: HATPase_c" amino acids 694 to 808 (115 residues), 98.4 bits, see alignment E=8.7e-32 PF00072: Response_reg" amino acids 833 to 947 (115 residues), 84.3 bits, see alignment E=1.8e-27 PF01627: Hpt" amino acids 985 to 1067 (83 residues), 46 bits, see alignment 1.3e-15

Best Hits

KEGG orthology group: K07679, two-component system, NarL family, sensor histidine kinase EvgS [EC: 2.7.13.3] (inferred from 78% identity to pba:PSEBR_a3905)

Predicted SEED Role

"Hybrid sensory histidine kinase in two-component regulatory system with EvgA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1092 amino acids)

>PFR28_02865 Sensor histidine kinase RcsC (Pseudomonas sp. RS175)
MQLLRTGLVALLLGSLSVMPQAGAAPEWLEVLGRSHVDDYSVSLEAADQAWLRQKGTLRL
GASAPDYAPFSITGNGRDYEGLTADYAQLLGQLLQVQVRVLRYPSRAESLQALREGEIDL
LGTANGFEADDPALVMSRAYADDLPTLVARIDDSQDLPPDLAGKRLAMLYHYLPPERVEA
FYPDATLQLYPSTLSAIGAVAFGQADVYLGDSISSNYLISKNYLNNVQLADFSRLEVQPF
AFALRRDNARLLRLVNAALQAIPTGENMVIQRRWSAGGASMPGQQALHFSVDEQRWLEAH
PRIRVVIDENFLPLTFTDEQGHLRGISADVLARISLRTGLKFDIQRGKSVYDLIDQVRSG
RADVLAAITISTGAEDDDTLRFTRPYLTNPFVLVTSLKGKAPLTLDQMEGKRLALVRGNV
LQPYLQETFAKVQLVPAQNVPEAMAMVAAGTVDGAVNSLISARYMISRQYRDRLQVTSTV
GTLPARIALATHRDASQLHGILDKALLGISPEEMDELTNRWRSEVVIDDSYWLRNRTTIV
QGFAIAALLLLLALGWVAYLRRLLEQLRVAKASADDANRAKTTFVATMSHEIRTPMNAVI
GMLELAQKKAEQGVVDREAIEVASGAAHGLLDLIGDILDIARIESGRLSLAPERANLRQL
VESVARMFEGLARHKHLDLPQDLGEAANRDVLIDPLRFKQIVSNLLGNAIKFTDRGQVRL
SLQAEPGPDGERLDIRLRIEDTGCGIARQDQEQLFRPFTQAPHSRQSARSGAGLGLVISR
TLCEMMGGSLTLESAPGQGTQVEVRLSLPLLDALAPVPKLEVEPATSARRLDILVVDDHP
ANRLLLSRQLSYLGHRVEVAEDGVQGLRAWRDGHFDAVISDCNMPSMNGYELARAIRADE
RIRGQAPAVILGFTANAQREEKDRCVEAGMDDCLFKPIGLTVLRARLEDVIDARAGGTES
AAAIDDLDLSSLEELTGGDTASLRELLGTLLRSNTDDEAHLVHLADRRDVPGLADLAHRI
KGGARMIQAQHLIAACEALEDACRSADASSVASAVEDVRQAMEQLAQRLQDHLAGGTARA
GPRSDTGGFLRE