Protein Info for PFR28_02508 in Pseudomonas sp. RS175

Annotation: Multidrug export protein MepA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 9 to 30 (22 residues), see Phobius details amino acids 50 to 74 (25 residues), see Phobius details amino acids 86 to 110 (25 residues), see Phobius details amino acids 130 to 150 (21 residues), see Phobius details amino acids 161 to 182 (22 residues), see Phobius details amino acids 189 to 210 (22 residues), see Phobius details amino acids 239 to 264 (26 residues), see Phobius details amino acids 284 to 306 (23 residues), see Phobius details amino acids 319 to 341 (23 residues), see Phobius details amino acids 358 to 378 (21 residues), see Phobius details amino acids 385 to 405 (21 residues), see Phobius details amino acids 416 to 437 (22 residues), see Phobius details PF01554: MatE" amino acids 16 to 175 (160 residues), 100.5 bits, see alignment E=4.2e-33 amino acids 245 to 406 (162 residues), 98.4 bits, see alignment E=1.8e-32 TIGR00797: MATE efflux family protein" amino acids 16 to 417 (402 residues), 213.7 bits, see alignment E=2.1e-67

Best Hits

Swiss-Prot: 41% identical to YOEA_BACSU: Probable multidrug resistance protein YoeA (yoeA) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 91% identity to pba:PSEBR_a3437)

Predicted SEED Role

"Na+ driven multidrug efflux pump"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>PFR28_02508 Multidrug export protein MepA (Pseudomonas sp. RS175)
MPAQPLWKTYLLFLTPMVLSNFLQSMSGTFNSIYIGQMLGTQALAAVSGMFPVLFFFIAL
VIGLGAGAGVLIGQAYGAQETGKVKAIAGATLLLGAIIGLVAAVLGSVFARDALHGLGTP
ADVLDDAVSYARVMMWILPILLVFVLFTQLLRGVSDTLSPLLALLVSTGVGLVLTPALIR
GWLGLPQMGIQSAAMAGLVGTTAAMAMLAWRLTRRQNVLAPDRALFAAMRLDRAILAKVL
RIGLPTGLQMVVISLSELVILALVNRHGSEATAAYGAVTQIVNYVQFPALSIAITASILG
AQAIGAGRLERLGPILRTGLIINLWLTGGLVLLGYLLSHWLLGLFITDPTARVQAEHLLH
IMLWSLLVFGFQAIVGGIMRAGGTVLLPVTISIFCIVGVQVPAAYLLDAYFGLQGVWMAF
PVAYLSMLLLQTLYYKLVWLHQPIERLV