Protein Info for PFR28_02506 in Pseudomonas sp. RS175

Annotation: 'Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase' transl_table=11

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14793: DUF4478
  • amino acids 10 to 116 (107 residues), 146.8 bits, E=4.3e-47
• PF03641: Lysine_decarbox
  • amino acids 196 to 331 (136 residues), 96.6 bits, E=2.9e-31
• PF11892: PpnN_C
  • amino acids 336 to 456 (121 residues), 184.8 bits, E=1.1e-58

Best Hits

Swiss-Prot: 57% identical to PPNN_ECOL6: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase (ppnN) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K06966, (no description) (inferred from 99% identity to pba:PSEBR_a3435)

MetaCyc: 57% identical to nucleotide 5'-monophosphate nucleosidase (Escherichia coli K-12 substr. MG1655)
AMP nucleosidase. [EC: 3.2.2.4]; Inosinate nucleosidase. [EC: 3.2.2.4, 3.2.2.12]; Pyrimidine-5'-nucleotide nucleosidase. [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]; 3.2.2.- [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]

Predicted SEED Role

"Decarboxylase family protein"

MetaCyc Pathways

• adenosine nucleotides degradation III (1/1 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.4

Use Curated BLAST to search for 3.2.2.10 or 3.2.2.12 or 3.2.2.4

Protein Sequence (457 amino acids)

>PFR28_02506 'Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase' transl_table=11 (Pseudomonas sp. RS175)
MTQRHVINASVSPKGSLETLSQREVQQLSEAGSGSIYTLFRQCALAILNTGAHVDNAKTI
LEAYKDFEIRIHQQDRGVRLELLNAPADAFVDGEMIASTREMLFSALRDIVYTENELDSQ
RIDLSTSQGISDYVFHLLRNARTLRPGVEPKIVVCWGGHSINTEEYKYTKKVGHELGLRS
LDICTGCGPGVMKGPMKGATIAHAKQRIHGGRYLGLTEPGIIAAEAPNPIVNELVILPDI
EKRLEAFVRVGHGIIIFPGGAGTAEEFLYLLGILMHPANQDLPFPVVLTGPKSAAPYLDQ
LHAFVGATLGEAAQKHYQIIIDDPAEVARQMTQGLKAVKQFRRERNDAFHFNWLLKIDEG
FQRPFDPTHANMASLGLRRDLPAHELAANLRRAFSGIVAGNVKDKGIRLIEEHGPYEIHG
DAAIMEPLDRLLKAFVAQHRMKLPGGAQYVPCYRVVT