Protein Info for PFR28_02437 in Pseudomonas sp. RS175

Annotation: 6-deoxy-6-sulfogluconolactonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 90% identity to pba:PSEBR_a3372)

Predicted SEED Role

"Gluconolactonase (EC 3.1.1.17)" in subsystem Entner-Doudoroff Pathway (EC 3.1.1.17)

MetaCyc Pathways

Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
glucose and glucose-1-phosphate degradation (4/5 steps found)
glucose degradation (oxidative) (4/5 steps found)
sorbitol biosynthesis II (2/3 steps found)
Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)
L-ascorbate biosynthesis IV (animals, D-glucuronate pathway) (3/6 steps found)
L-ascorbate biosynthesis VI (plants, myo-inositol pathway) (1/4 steps found)
L-ascorbate biosynthesis VIII (engineered pathway) (2/7 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.17

Use Curated BLAST to search for 3.1.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (301 amino acids)

>PFR28_02437 6-deoxy-6-sulfogluconolactonase (Pseudomonas sp. RS175)
MQAELIVDARNAVGESPVWVPEENALYWVDIPSGGLQCWNAGTGQLKGWNTPEMLACIAR
HQDGGWVAGMESGFFRLHPNDDDTLDSELCANVEHSRPDMRLNDGRCDRQGRFWAGSMVL
NMGANVAEGRMYRFEAGQRSPVEAQLSGFIVPNGLGFSPDGRTMYLSDSHPLIQQIWAFD
YDIDSGTPTNRRLFVDMMPLAGRPDGAAVDAEGCYWICANDAGLIHRFTPDGRLDRSLEV
PVKKPTMCAFGGSRLDTLFVTSIRLGDDSDPQSLAGGVFALNPGVKGLAEPAFGGLKHAA
N