Protein Info for PFR28_02340 in Pseudomonas sp. RS175

Annotation: Periplasmic trehalase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 541 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF01204: Trehalase" amino acids 56 to 533 (478 residues), 576.7 bits, see alignment E=2.7e-177

Best Hits

Swiss-Prot: 67% identical to TREA_PSEAE: Periplasmic trehalase (treA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01194, alpha,alpha-trehalase [EC: 3.2.1.28] (inferred from 84% identity to pba:PSEBR_a2379)

MetaCyc: 56% identical to periplasmic trehalase (Escherichia coli K-12 substr. MG1655)
Alpha,alpha-trehalase. [EC: 3.2.1.28]

Predicted SEED Role

"Trehalase (EC 3.2.1.28); Periplasmic trehalase precursor (EC 3.2.1.28)" (EC 3.2.1.28)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (541 amino acids)

>PFR28_02340 Periplasmic trehalase (Pseudomonas sp. RS175)
MRPLPFTSLCFTAVLCAACSSQPAATWSYMDARDRVTQTPDQAYPELFEAVQRGAIFTDQ
KHFVDALPKRDPATIRADYLARRDAAGFDLKAFVEDNFIESGEALSPAPKPGAPIKEHID
KLWPVLSRTYRQVPPYSSLLPLPEPYVVPGGRFREMYYWDSYFTMLGLEQSGDKAQVRHM
TDNFAYMIDTYGHIPNGNRIYYLSRSQPPFFAYMVELQAHIEGSQAYGRYLPQLQKEYAY
WMQDSQTLKPGTAARHVVKLADGSVLNRYWDASPTPRQESWLQDVKTAAQAPDRPREEVW
RDLRAGAESGWDFSSRWLGDGKDLASIRTTSIVPVDLNSLLYHLERTIAKACETTRNAPC
VQAYGQRAEQRQRAIEKHLWNEAAGYYVDYDWQENRQRPDLTAATLFPLYTGLAGAERGH
RTGEAVRKGLLRPGGIATTQVDNGQQWDEPNGWAPLQWVAVQGLDRYRQTALAGQIGQRF
LQQVRKLYDAEDKLVEKYDVSGAGQGGGGGEYELQDGFGWTNGVTLKLLEKYGENTGAGA
R