Protein Info for PFR28_02264 in Pseudomonas sp. RS175

Annotation: Dimodular nonribosomal peptide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 614 PF00501: AMP-binding" amino acids 27 to 379 (353 residues), 285.8 bits, see alignment E=7.2e-89 TIGR01733: amino acid adenylation domain" amino acids 48 to 451 (404 residues), 418.6 bits, see alignment E=1.2e-129 PF13193: AMP-binding_C" amino acids 435 to 514 (80 residues), 41 bits, see alignment E=4.4e-14 PF00550: PP-binding" amino acids 543 to 606 (64 residues), 35.5 bits, see alignment E=1.5e-12

Best Hits

KEGG orthology group: None (inferred from 78% identity to psb:Psyr_2611)

MetaCyc: 78% identical to L-threonine--[L-threonyl peptide-carrier protein] ligase (Pseudomonas syringae pv. syringae)
RXN-19453 [EC: 6.2.1.70]

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (614 amino acids)

>PFR28_02264 Dimodular nonribosomal peptide synthase (Pseudomonas sp. RS175)
MPIKNTGGSVNPAPAPITPGALLHEIFAARAREFPERTAVSDATRSLSYAQLDAASSQLA
ARLRQEGVKDGMLVGMCLARSVDLVISLLGILKAGGAYVPVDPQYPGKRVEHIVRDSGMS
LLIGDLADLPQSSSLRVLPLAELLAGPTLEPVAEAERRDPDNAVAYVIYTSGSTGEPKGV
LVAHGNVSRLLESTQREYAFTEHDVWSMFHSIGFDFSVWEIWGALAHGGHVAVVPYDVSR
SPAAMLQWLAGQGVTVLSQTPSAFRGLDEADRQAGAPLALRYVVFGGEALPATVLRPWVE
RHGDQKPALINMYGITEATVHTTFKRVLAQDLESNAMVSIGAPLDGWRLNLLDANQQPVP
QGTAAELYIEGAGVAMGYLNRPALNAERFVQLPGGSGRAYRTGDLLVLGDDGEYRYAGRC
DEQLKIRGFRIEPGEVEACLQSSPAVAAAHVAGHDFGDGDWRLVTYVVPTQGVQAWNEQT
RSEVAALVAASLPDYMRPSAYIPLAELPVTAHGKIDRQRLPAPESIAATVHHTSEGLTEQ
EQFVLKVWSQDIGLKNIGLNDDFFDFGGTSLALIRSLSTLKAHYQVNLDPGVLANGATAK
VLADCIQRSLVQAH