Protein Info for PFR28_02195 in Pseudomonas sp. RS175

Annotation: Multidrug resistance protein MdtA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 70 to 396 (327 residues), 241.2 bits, see alignment E=6.8e-76 PF16576: HlyD_D23" amino acids 93 to 315 (223 residues), 37.7 bits, see alignment E=2.1e-13 PF13533: Biotin_lipoyl_2" amino acids 93 to 140 (48 residues), 52 bits, see alignment 7.3e-18 PF13437: HlyD_3" amino acids 204 to 305 (102 residues), 28.9 bits, see alignment E=2.3e-10

Best Hits

Swiss-Prot: 48% identical to MDTA_KLEP7: Multidrug resistance protein MdtA (mdtA) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K07799, putative multidrug efflux transporter MdtA (inferred from 91% identity to pba:PSEBR_a2800)

MetaCyc: 50% identical to multidrug efflux pump membrane fusion protein MdtA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-353; TRANS-RXN-92

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (433 amino acids)

>PFR28_02195 Multidrug resistance protein MdtA (Pseudomonas sp. RS175)
MQSSVSRNSRRWLLSLFIVLVVAVVAWKFWPAGTDPKQGAGQKAAQGHTGRSGSMRPGFG
GATGPVPVRVAPAVTGDFALYYKALGTVTALNTINVRSRVSGELVKIAFEEGQMVKAGDL
LAEIDPRPYQNALLQAEGTLLQNQAQLKNAQVDLERYRGLYAEDSIAKQTLDTAAALVGQ
YQGTVKTNQAAVNDAKLNLEFTRIRAPISGRVGLRQLDIGNLVAANDTTALAVITQTQPI
SVVFTLPESNLDTVLARYRSGAKLPVEAWDRGDVKLQASGVLQSLDNQIDTTTGTLKFKA
RYDNRDQSLFPNQFVNVRLLADTLKGVVLAPTAAIQFGTNGTFVYALDGDRKVTIKTLKI
GPSDGSNTVVTEGLAAGERVVLEGTDRLKEGSEVEVVNDSQDVPTTPTEHLQGKTAAGPS
EPAVVDKAKKGGA