Protein Info for PFR28_02147 in Pseudomonas sp. RS175

Annotation: 4-alpha-glucanotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 692 TIGR00217: 4-alpha-glucanotransferase" amino acids 112 to 661 (550 residues), 337.8 bits, see alignment E=7.5e-105 PF02446: Glyco_hydro_77" amino acids 158 to 661 (504 residues), 380.6 bits, see alignment E=6e-118

Best Hits

KEGG orthology group: K00705, 4-alpha-glucanotransferase [EC: 2.4.1.25] (inferred from 94% identity to pba:PSEBR_a3008)

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (692 amino acids)

>PFR28_02147 4-alpha-glucanotransferase (Pseudomonas sp. RS175)
MSDAQLEILAGRAGLAVDWIDANGRAQKVSPAVLRSVLTGLGHPAGTAQEIDASLLQLQQ
DQQNHRLPPLITADVGVSVDLSRYFEGSTPCEIRLEDGATLNLKLDADARLPGMVPVGYQ
HVSIQDQHFTLAVAPARCYSVADAVDDPTPRAWGLSAQLYALRRPGDGGFGDTQALEELA
RTAGERGADALAISPIHAMFSSDTGRYSPYSPSSRLFLNSLYAAPGTILGERALRTAIDA
TGLTNQLRHLEDQPLVDWPVAAEAKQKILRALYDGFNQGEHPLHEDFASFRHSSGEALEN
HCRFEALQAERAARGESLDWREWPEQWRNPRSPTLARFAEENADEIGFYAFCQWLIARCL
ERAQSAAKSSGMGIGLIADLAVGADGAGSQAWSRQDELLASLTVGAPPDILNRSGQGWGI
SAFSPEGLVRNGFRAFIEMLRANFAHAGGLRIDHVMGLQRLWVIPNGAPPTDGAYLYYPV
DDLLRLLALESHRHQAIVLGEDLGTVPDGLREKLSARAILGMRVLLFEQDNTHFKPILDW
PDNALATTSTHDLPTLNGWWHGHDIDWNARLNLIDSHTEMDWRRHREREREGLRHVLNQD
PQNFREEHRETDQVLDASVRFLGHTRAPLVLLPLEDALGIDEQANLPGTIDTHPNWRRRL
PCESQALLDGPDAARRLEILACARLQANERDR