Protein Info for PFR28_02059 in Pseudomonas sp. RS175

Annotation: Oxygen sensor protein DosP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 846 TIGR00229: PAS domain S-box protein" amino acids 11 to 132 (122 residues), 86.2 bits, see alignment E=1.9e-28 amino acids 134 to 250 (117 residues), 54.5 bits, see alignment E=1.2e-18 PF08448: PAS_4" amino acids 15 to 127 (113 residues), 26.6 bits, see alignment E=2.8e-09 amino acids 140 to 248 (109 residues), 38.5 bits, see alignment E=5.5e-13 PF00989: PAS" amino acids 15 to 122 (108 residues), 35.2 bits, see alignment E=5e-12 amino acids 135 to 235 (101 residues), 33.5 bits, see alignment E=1.6e-11 PF13426: PAS_9" amino acids 20 to 124 (105 residues), 45.5 bits, see alignment E=3.5e-15 amino acids 144 to 245 (102 residues), 45.5 bits, see alignment E=3.4e-15 PF13188: PAS_8" amino acids 135 to 188 (54 residues), 29.9 bits, see alignment 1.7e-10 PF13185: GAF_2" amino acids 265 to 408 (144 residues), 37.2 bits, see alignment E=1.5e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 418 to 577 (160 residues), 148.5 bits, see alignment E=1.5e-47 PF00990: GGDEF" amino acids 420 to 574 (155 residues), 156.8 bits, see alignment E=1.9e-49 PF00563: EAL" amino acids 596 to 831 (236 residues), 245.1 bits, see alignment E=3.1e-76

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (846 amino acids)

>PFR28_02059 Oxygen sensor protein DosP (Pseudomonas sp. RS175)
MPDHKDLSQVFIQTLEQSVDSVVVIDSHNSIILFNEAAQKLWGYSPDEVMGRNVDLLVPQ
KIRPHHDGFIDANRRTGINKIVGTSRTVPIERKDGSRRWGAMSISRVETQGQILYTAFIK
DITQQHEEQHRLYLLSLVVDTTENAILITDASWRIIFVNDGFVQMFGYGAQEAAGKTPIE
LLAPHLEHRQVAGIRHQVARHHVYHGQNYGYHREGPRVWCNITSHSVHDAHGQLANTVSV
LTDVTHTRMHEVLRHKILEAMVREESLKTVLNLACLEVQRIAPEVTASVLHVDDRGCLQT
LAAPGLPSGFCMALDGTAIGAGLDALAATVFSGEAATISELDAHPFWARFKEQKQPLGLT
RCWATPVKSNDGRVLGLFAFYYRDSGQPSRLHQLLVDACIHLCALALEREESRQHIRKLA
FYDELTGLANRNLLHARAEQAIADASRHQSNLAVVFIDMDRFKQVNDSLGHPAGDEFLRL
VAHRLSEDRREHDIVSRLSGDEFVLVLPQCDSAQAIDLIEKLRARLNVPSQVCGVTLRPS
ASIGVSLFPEDGCTMEVLLHRADMAMYQAKTRARGSVCFFNDGLSQLTQCRLRLEAALRE
AMDQRSLHLAYQPQIDLGRSALYGVEVLARWTHPQLGEISPARFIALAQECGLISELGLW
VLSEACRQLAAWRKQGLLVPAVSVNMSPTHFHDRDLPTILMRTLRAHGLQARDLTLEITE
NVLMDINPDTLTTIEQVHALGITLAMDDFGTGYSSLSYLRRLPIQELKLDRSFVHDLERD
TTSQALSEAVIRIGESLQMRVVAEGVETLAQQQVLQNQGYRIVQGFLYSRPLVAEEIGPW
IRARYV