Protein Info for PFR28_01839 in Pseudomonas sp. RS175
Annotation: Long-chain-fatty-acid--CoA ligase FadD15
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K12508, feruloyl-CoA synthase [EC: 6.2.1.34] (inferred from 92% identity to pba:PSEBR_a3134)MetaCyc: 48% identical to 4-coumarate-CoA ligase (Rhodopseudomonas palustris CGA009)
4-coumarate--CoA ligase. [EC: 6.2.1.12]
Predicted SEED Role
"Trans-feruloyl-CoA synthase (EC 6.2.1.34)" (EC 6.2.1.34)
MetaCyc Pathways
- ferulate degradation (3/3 steps found)
- 4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
- umbelliferone biosynthesis (2/3 steps found)
- 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
- 4-coumarate degradation (aerobic) (2/5 steps found)
- naringenin biosynthesis (engineered) (1/4 steps found)
- xanthohumol biosynthesis (1/4 steps found)
- chlorogenic acid biosynthesis II (1/5 steps found)
- flavonoid biosynthesis (1/5 steps found)
- phaselate biosynthesis (1/5 steps found)
- 4-coumarate degradation (anaerobic) (1/6 steps found)
- chlorogenic acid biosynthesis I (1/8 steps found)
- rosmarinic acid biosynthesis I (2/10 steps found)
- avenanthramide biosynthesis (1/9 steps found)
- curcuminoid biosynthesis (1/10 steps found)
- coumarins biosynthesis (engineered) (3/13 steps found)
- flavonoid di-C-glucosylation (3/15 steps found)
- superpathway of rosmarinic acid biosynthesis (2/14 steps found)
- monolignol biosynthesis (1/15 steps found)
- suberin monomers biosynthesis (3/20 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Phenylpropanoid biosynthesis
- Ubiquinone and menaquinone biosynthesis
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.2.1.12 or 6.2.1.34
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (621 amino acids)
>PFR28_01839 Long-chain-fatty-acid--CoA ligase FadD15 (Pseudomonas sp. RS175) MSSESRSSSRPDAGRYRQVSIGHPAVEVSEAQGILHMRSLEALEPLPARLLDRLVHWARV HPQQTFIAARVAGGDWRRVSYAEMLDSVRAIAQGLLGYGLSAERPLVLLSGNDIEHLQMA FGAMYAGIPYCPVSPAYSLLSQDFAKLRHVCDLLQPGLVYVSDAAPFERAINAVLPAGVP LITARGEIPGRARTRFASLLAQPGGVEAERAFAATGPDSIAKFLFTSGSTKLPKAVITTQ RMLCANQQMLLQTFPVFGEEPPVLVDWLPWNHTFGGSHNVGIVLYNGGTFYLDDGKPTAQ GFAETLRNLKEISPTAYLTVPKGWEELVSALEQDGELRERFFDRISLFFFAAAGLSQSTW DRLDKVAEQHCGERIRMMAGLGMTEASPSCTFTTGPLSVAGYIGLPAPGCEVRLVPVDGK FEGRFRGPHIMPGYWRSPQQTAEVFDEDGFYCSGDAIKLADPGNPQLGLMFDGRIAEDFK LSSGVFVSVGPLRNRAVLEGTPYVQDLVVTAPDRECLGALVFPRLPECRRLSGLGSAASD AQVLASAPVRQWFADWLQRLNRQASGNASRVEWIALLDEPASIDRGEITDKGSLNQRAVL QWRAAKVEALYRGEDPAILRA