Protein Info for PFR28_01633 in Pseudomonas sp. RS175
Annotation: Respiratory nitrate reductase 2 beta chain
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 74% identical to NARY_ECOLI: Respiratory nitrate reductase 2 beta chain (narY) from Escherichia coli (strain K12)
KEGG orthology group: K00371, nitrate reductase 1, beta subunit [EC: 1.7.99.4] (inferred from 98% identity to pba:PSEBR_a3147)MetaCyc: 90% identical to respiratory nitrate reductase alpha subunit (Stutzerimonas stutzeri)
RXN-11236 [EC: 1.7.5.1]
Predicted SEED Role
"Respiratory nitrate reductase beta chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)
MetaCyc Pathways
- nitrate reduction I (denitrification) (5/5 steps found)
- NADH to nitrate electron transfer (2/2 steps found)
- nitrate reduction IX (dissimilatory) (2/2 steps found)
- nitrate reduction VII (denitrification) (4/5 steps found)
- nitrate reduction III (dissimilatory) (1/2 steps found)
- nitrate reduction VIII (dissimilatory) (1/2 steps found)
- nitrate reduction VIIIb (dissimilatory) (1/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.7.99.4
Use Curated BLAST to search for 1.7.5.1 or 1.7.99.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (512 amino acids)
>PFR28_01633 Respiratory nitrate reductase 2 beta chain (Pseudomonas sp. RS175) MKIRSQIGMVLNLDKCIGCHTCSITCKNVWTSREGMEYAWFNNVESKPGIGYPKEWENQD KWKGGWVRNADGSINPRIGGKFRVLANIFANPDLPSLDDYYEPFDFDYQHLHTAPLGEHQ PTARPRSVVSGKRMEKIEWGPNWEEILGTEFAKRRKDKNFDKVQADIYGEYENTFMMYLP RLCEHCLNPACAASCPSGAIYKREEDGIVLIDQEKCRGWRMCISGCPYKKIYFNWKSGKS EKCIFCYPRIEAGMPTVCAETCVGRIRYLGVLLYDADRISEVASTADEKDLYEKQLEIFL DPNDPAVIRQALADGVPQSVIDSAQRSPVYKMAVDWKLALPLHPEYRTLPMVWYVPPLSP IQNAAAAGTVGMNGVIPDVDSLRIPLRYLANMLTAGDEKPVRHALKRLLAMRAYKRSQQV DGVQDLQVLADVGLSVNQVEEMYRYLAIANYEDRFVVPSAHREDAMSDAFAERSGCGFSF GSGCSGSSDTNMFGGKKANRRDVIKTVQLWEE