Protein Info for PFR28_01549 in Pseudomonas sp. RS175

Annotation: Oligopeptide-binding protein AppA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF00496: SBP_bac_5" amino acids 103 to 506 (404 residues), 224.4 bits, see alignment E=1.3e-70

Best Hits

Swiss-Prot: 43% identical to Y3691_BRUME: Putative binding protein BMEII0691 (BMEII0691) from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

KEGG orthology group: K13893, microcin C transport system substrate-binding protein (inferred from 95% identity to pba:PSEBR_a3289)

Predicted SEED Role

"ABC transporter, periplasmic substrate-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (617 amino acids)

>PFR28_01549 Oligopeptide-binding protein AppA (Pseudomonas sp. RS175)
MKPLRALLLQAGGLMFAGLACAAPQHALTLYNEPPKYPADFKHFDYVNPDAPKGGIFRQG
GFGGFDSLNPFISKGVPADDIGLIYDTLAKQGLDEPFTEYGLIAGKIEKAPDNAWVRFYL
RPEARFNDGHPVRAEDVVFSFQTLTKDGAPMFRGYYNDVAEVIAEDPLKVLFKFKHTNNR
ELPLILGQLPVLPKHWWAERDFNKGNLEIPLGSGPYRVAEVKAGRSIRYERVKDYWGKDL
PVNRGFYNFDVLSTDYYRDNTVAVEALKAGQFDFWLEMTAKNWANAYNIPAVTEGRLIKE
QIPNGNPTGMQGFVYNLRRPVFQDVRVRKALSLLLDFEWTNKQLFNGAYARTRSYFENSE
MAATGLPGEDELKILEPLRGKIPEQVFTEAFQPSMCDGSGMIRDQQRKAYQLLQEAGWRI
VDDRMVDAQGKPVVLEFLLAQTEFERVLLPFKRNLSDLGIELVIRRVDVSQYINRVRSRD
FDLVVGSFPQSSSPGNEQREFWMSAAADKPGSRNTMGLKDPAVDQLVEQLINADSRKSLV
AHARALDRVLQWGYYVIPNWHIKTWRVAYWNHIGHPKVTPTYDIGTTTWWVKPDTKPAEE
VEHVIEKQNNAASASVE