Protein Info for PFR28_01546 in Pseudomonas sp. RS175

Annotation: putative ABC transporter ATP-binding protein YejF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 536 PF00005: ABC_tran" amino acids 27 to 186 (160 residues), 108.5 bits, see alignment E=1.3e-34 amino acids 303 to 453 (151 residues), 111.5 bits, see alignment E=1.6e-35 PF08352: oligo_HPY" amino acids 237 to 269 (33 residues), 25.1 bits, see alignment (E = 5.5e-09) amino acids 504 to 528 (25 residues), 22.9 bits, see alignment (E = 2.7e-08)

Best Hits

Swiss-Prot: 58% identical to YEJF_ECOLI: Uncharacterized ABC transporter ATP-binding protein YejF (yejF) from Escherichia coli (strain K12)

KEGG orthology group: K13896, microcin C transport system ATP-binding protein (inferred from 96% identity to pba:PSEBR_a3292)

MetaCyc: 58% identical to putative oligopeptide ABC transporter ATP binding subunit YejF (Escherichia coli K-12 substr. MG1655)
3.6.3.23-RXN [EC: 7.4.2.6]

Predicted SEED Role

"Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)" in subsystem ABC transporter oligopeptide (TC 3.A.1.5.1) (TC 3.A.1.5.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (536 amino acids)

>PFR28_01546 putative ABC transporter ATP-binding protein YejF (Pseudomonas sp. RS175)
MNQDNLIEVRDLAVEFIAGHHHQRVVEGVSFDIKRGETLALVGESGSGKSVTAHSILRLL
PYPLAHHPTGTIQYAGQDLLSLKEKTIRHIRGNRIAMIFQEPMTSLNPLHSIGKQINEVL
GIHKGLTGKVATRRTLELLELVGIPEPHKRLKALPHELSGGQRQRVMIAMALANEPELLI
ADEPTTALDVTVQLKILDLLKQLQARLGMSLLLISHDLNLVRRIAHRVCVMQRGCIVEQA
SCEELFRAPQHPYTRELLAAEPSGNPAANVAGPPLLQVEDLKVWFPVKKGLFKRTVDYIK
AVDGIRFSLPQGQTLGIVGESGSGKSTLGLAILRLIASQGGIRFEGKQLDSLSQQQVRPL
RREMQVVFQDPFGSLSPRMCVGQIVGEGLRIHKIGTEAEQEQAIIAALEEVGLDPETRNR
YPHEFSGGQRQRIAIARALVLKPALILLDEPTSALDRTVQRQVVELLRSLQSKYNLTYLF
ISHDLAVVKALSHQLMVVKHGQVVEQGDARSLFAAPQHPYTQQLMEAAFLAPTTAE