Protein Info for PFR28_01394 in Pseudomonas sp. RS175

Annotation: Acetyl-coenzyme A synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 651 transmembrane" amino acids 317 to 336 (20 residues), see Phobius details TIGR01217: acetoacetate-CoA ligase" amino acids 4 to 649 (646 residues), 869.4 bits, see alignment E=7.2e-266 PF16177: ACAS_N" amino acids 36 to 91 (56 residues), 49.2 bits, see alignment 5.8e-17 PF00501: AMP-binding" amino acids 101 to 469 (369 residues), 162.9 bits, see alignment E=1.6e-51 PF13193: AMP-binding_C" amino acids 545 to 613 (69 residues), 25.7 bits, see alignment E=2.8e-09

Best Hits

Swiss-Prot: 46% identical to AACS_MOUSE: Acetoacetyl-CoA synthetase (Aacs) from Mus musculus

KEGG orthology group: K01907, acetoacetyl-CoA synthetase [EC: 6.2.1.16] (inferred from 90% identity to pba:PSEBR_a1970)

Predicted SEED Role

"Acetoacetyl-CoA synthetase (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Polyhydroxybutyrate metabolism (EC 6.2.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.16

Use Curated BLAST to search for 6.2.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (651 amino acids)

>PFR28_01394 Acetyl-coenzyme A synthetase (Pseudomonas sp. RS175)
MSDILWQPSPERIAQSRMNAFRLFVNGRHHLQLDDYPALHAWSIDQREAFWQAIVDFFEI
HFHHPPTAVLVEGPKMPSARWFPGATLNFAEHLLRRRDPSVAVVAIDERGDQERLTWAEL
AAHVAGLQRSLQAAGVCQGDRVAACMPNTWQTLVGMLATTSLGAIWSCSSPDFGTQGIID
RFGQIEPKVLITCAGYRYAAKTIDQRPKVNEILERLPSLRQLIVLPYATPDTRVADFRTQ
ASVALWDDFYRPGGDPVFVSVPFDHPLYILYSSGTTGVPKCIVHGTGGVLLQHMKEHGLH
VDLGPEDRLFYYTTCGWMMWNWLVSALAVGSTVVLYDGSPFHPGPERLPDLIDQQRISVF
GTSPKFLAALENQGLAPRRSHDLRSLKTILSTGSALAPHSYDYVYRDFKTDVCLASMSGG
TDIVSCFVNGNPLAPVRRGEMQGKSLGMAVQVWNETASPVIGEKGELVCTRHFPAMPIGL
WNDPRQEKLRASYFAQFPGVWSQGDYAEELPHGGWLIHGRSDAVLNPGGVRIGTAEIYRQ
VEKVEQVLDSVAIGQQWQGDVRVVLFVRLRDGVQLDEPLEQRIRQVIRANTTPRHVPAKI
LAVSDIPRTISGKVVELAVRNVVHGEAVKNTDALANPEALEQFRDRPELAS