Protein Info for PFR28_01377 in Pseudomonas sp. RS175

Annotation: Aerobic cobaltochelatase subunit CobN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1257 TIGR02257: cobaltochelatase, CobN subunit" amino acids 1 to 1254 (1254 residues), 1730.1 bits, see alignment E=0 PF02514: CobN-Mg_chel" amino acids 138 to 1241 (1104 residues), 1321.8 bits, see alignment E=0

Best Hits

KEGG orthology group: K02230, cobaltochelatase CobN [EC: 6.6.1.2] (inferred from 58% identity to bac:BamMC406_1576)

Predicted SEED Role

"CobN component of cobalt chelatase involved in B12 biosynthesis" in subsystem Coenzyme B12 biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1257 amino acids)

>PFR28_01377 Aerobic cobaltochelatase subunit CobN (Pseudomonas sp. RS175)
MHLLRTQPGGFVSDDNIADLGQTPAELVILCSGDSSLALLAEAAKQLPGDYPEFRLANPM
QVQNHASVDLYFEDVLRHAKVILLSLHGGIGYWRYGIERLMALAGRGVKLILVPGDDRPD
PELSDLSNVPVEDRDRLWQFLRQGGLGNALDLFHNLASQWFGRDYPWGEPQALPRTAIYH
PTHNQASLADWRADWQAGQPVAAVLFYRSHLQAANTAFIDVFCQRLQAAGLNPLPMAVAS
LKEPGCLAMVQDWLDEAEAGVILNTTGFAQSSPEAPHLRPFRRNVPVIQAICAQDNEPGW
RASEQGLGPRDLAMHIALPELDGRIISRPISFKDLAWRSELSQSDVVCYRAVPERMDFVA
ELARRWTDLARLPNAEKRVALILANYPTRDGRIGNGVGLDTPAAALNILRALHAEGYPLP
AQLPASGTALIQQLLGGVSNDLDSLDLRPCHQSLSLDAYRAMFDALPESNRQAVLERWGT
PDKDPMFRQGRLMIAGLRLGLTFVGIQPARGYQVDPSAVYHDPDLVPPHGYLAFYFWLRH
AYGAHAVIHVGKHGNLEWLPGKGVGLSANCWPDALLGPLPNIYPFIVNDPGEGAQAKRRT
QAVIIDHLMPPLTRAETYGPLRNLELLADEYYEAQLLDPRRARELQRDILGLVRETHIDR
ELQLDAALDSDADAAIWLPRLDTYLCDLKESQIRDGLHVFGQSPAGRLRIDTLLALLRIP
RGDGKGAQSSLLRALARAFELGFDPLDCALAEPWGGGRPQALCQISDQAWRTVGDTRERL
ELFAAQLIERALDGAVEGLDAPGWETVSSIVEHLREVVAPRLDACGPAEMRGLLDALGGR
FVPAGPSGAPSRGRLDVLPTGRNFFSVDVRNLPTTTAWRIGFQSANLILERHLQDHGDHL
RQLGLSVWGTATMRTGGDDIAQAMALMGVRPVWATGSQRVDDFEILPLSLLDRPRVDVTL
RVSGFFRDAFANLIRLFDAAVQAVAALDEPDDMNPLAAKVRGEREALSASGLDPEVAARQ
AGWRIFGAKPGAYGAGVQGAVDGRLWQSREDLAEVYLNWGGYAYGGSDEGTAAREQFSRR
LSQVQAVLQNQDNREHDLLDSNDYYQFQGGMLAAVETLSGETAASYHGDHSQPDLPRIRT
LKEELNRVIRSRAVNPKWIDGVKRHGYKGAFELAATVDNLFAFDATTQLIDDHQYALLAD
AYLLDPHTQAFIRQHNPDALRDMTERMLEAQQRGMWQDPGEYREALENLLLDIEEDG