Protein Info for PFR28_01372 in Pseudomonas sp. RS175

Annotation: Precorrin-4 C(11)-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 76% identical to COBM_PSEAE: Precorrin-4 C(11)-methyltransferase (cobM) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K05936, precorrin-4 C11-methyltransferase [EC: 2.1.1.133] (inferred from 90% identity to pba:PSEBR_a1949)

MetaCyc: 48% identical to cobalt precorrin-4 (C11)-methyltransferase (Salmonella enterica enterica serovar Typhimurium)
RXN-8762 [EC: 2.1.1.271]

Predicted SEED Role

"Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 2.1.1.133)

MetaCyc Pathways

adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (13/13 steps found)
adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (13/15 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.133 or 2.1.1.271

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (248 amino acids)

>PFR28_01372 Precorrin-4 C(11)-methyltransferase (Pseudomonas sp. RS175)
MTVYFIGAGPGDPELITVKGQRLIRSCPVIIYAGSLVPSAVLDGHCAEQVVNSAELHLEQ
IVGLMETAHRKGQDVARVHSGDPSLYGAIGEQIRCLRALGIPFEIIPGVTATSACAALLG
SELTLPDISQSVILTRYADKSPMPAGEELGNLAQHRATMAIHLGVNHLEKIVDELLPHYG
GDCPIAVIHRATWPDQDWVVGTLADIAGKVQAKGLRRTALILVGRVLGNDVFSESSLYRA
GHAHLYRP