Protein Info for PFR28_01369 in Pseudomonas sp. RS175

Annotation: Methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 15 to 1202 (1188 residues), 1766.5 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 20 to 328 (309 residues), 319.2 bits, E=8.9e-99
• PF00809: Pterin_bind
  • amino acids 363 to 601 (239 residues), 200.8 bits, E=7.4e-63
• PF02607: B12-binding_2
  • amino acids 661 to 733 (73 residues), 81.9 bits, 7.9e-27
• PF02310: B12-binding
  • amino acids 749 to 842 (94 residues), 71.8 bits, 1.1e-23
• PF02965: Met_synt_B12
  • amino acids 942 to 1218 (277 residues), 404.5 bits, E=5.9e-125

Best Hits

Swiss-Prot: 87% identical to METH_PSEAE: Methionine synthase (metH) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 67% identity to aha:AHA_0432)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine salvage from L-homocysteine (2/3 steps found)
• folate transformations I (9/13 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-methionine salvage and degradation (6/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.13

Protein Sequence (1236 amino acids)

>PFR28_01369 Methionine synthase (Pseudomonas sp. RS175)
MSDRSARLYLLQQALKERILILDGGMGTMIQSYKLEEQDYRGKRFADWPSDVKGNNDLLV
LTRPDVIGSIEKAYLDAGADILETNTFNATQVSQADYGMQGLAYELNLEGARLARQVADA
KTLETPDKPRFVAGVLGPTSRTCSLSPDVNNPGYRNVTFDELVENYTEATKGLIDGGADL
ILIETIFDTLNAKAAIFAVQGVFEELGVELPIMISGTITDASGRTLSGQTTEAFWNSVAH
AKPISVGLNCALGASELRPYLEELSNKANTHVSAHPNAGLPNEFGEYDELPAETAKVIEE
FAQSGFLNIVGGCCGTTPAHIEAIAKAVAGYAPRQIPEIPRACRLSGLEPFTIDRNSLFV
NVGERTNITGSAKFARLIREDNYTEALEVALQQVEAGAQVIDINMDEGMLDSKKAMVTFL
NLIAGEPDISRVPIMIDSSKWEVIEAGLKCIQGKGIVNSISMKEGVEQFIHHAKLCKRYG
AAVVVMAFDEAGQADTEARKKEICKRSYDILVNEVGFPPEDIIFDPNIFAVATGIEEHNN
YAVDFINACAYVRDELPYALTSGGVSNVSFSFRGNNPVREAIHSVFLLYAIRAGLTMGIV
NAGQLEIYDQIPVELRDAVEDVILNRTPEGTDALLAIADKYKGDGSVKEAETEEWRNWDV
NKRLEHALVKGITTHIVEDTEESRLSFARPIEVIEGPLMAGMNIVGDLFGAGKMFLPQVV
KSARVMKQAVAHLIPFIEAEKGDKPEAKGKILMATVKGDVHDIGKNIVGVVLGCNGYDIV
DLGVMVPAEKILQVARDEKCDIIGLSGLITPSLDEMVHVAREMQRQDFHLPLMIGGATTS
KAHTAVKIEPKYSNDAVIYVTDASRAVGVATQLLSKELKPAFVEKARQDYQEVRERTANR
SARTERLSYSAAVAKKPQFDWSSYQPVKPTFTGTRVLEDIDLDVLAEYIDWTPFFISWDL
AGKFPRILTDEVVGEAATALYADAQAMLRKLIDEKLISARAVFGFWPANQVHDDDLEVYG
EDGKPMARLHHLRQQIIKTDGKPNFSLADFVAPKDSGVTDYVGGFITTAGIGAEEVAKAY
QDAGDDYNSIMVKALADRLAEACAEWLHQQVRKDYWGYAKDENLDNEALIKEQYTGIRPA
PGYPACPDHTEKATLFRLLDPQASEMKAGRSGVFLTEHYAMFPAAAVSGWYFAHPQAQYF
AVGKIDKDQVQSYTARKGQELSVTERWLAPNLGYDS