Protein Info for PFR28_01258 in Pseudomonas sp. RS175

Annotation: 1,4-dihydroxy-2-naphthoyl-CoA synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 13 to 262 (250 residues), 158 bits, E=2.8e-50
• PF16113: ECH_2
  • amino acids 17 to 193 (177 residues), 94.8 bits, E=7.5e-31

Best Hits

KEGG orthology group: K13766, methylglutaconyl-CoA hydratase [EC: 4.2.1.18] (inferred from 92% identity to pba:PSEBR_a1843)

MetaCyc: 74% identical to 3-methylglutaconyl-CoA hydratase (Pseudomonas putida)
Methylglutaconyl-CoA hydratase. [EC: 4.2.1.18]

Predicted SEED Role

"Methylglutaconyl-CoA hydratase (EC 4.2.1.18)" in subsystem Benzoate transport and degradation cluster or HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.2.1.18)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• ferrichrome A biosynthesis (2/5 steps found)

KEGG Metabolic Maps

• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.18

Protein Sequence (277 amino acids)

>PFR28_01258 1,4-dihydroxy-2-naphthoyl-CoA synthase (Pseudomonas sp. RS175)
MDNFNTLELLTDSRGVATLWLSRESKNNAFNAEMIRELILALDQVGSNPGLRFLLIRGRG
KHFSAGADLAWMQQSAELDYHTNLDDARELSELMYNLAKLKIPTLAVVQGAAFGGALGLI
SACDMAIGADDAQFCLSEVRIGLAPAVISPFVVQAIGERAARRYALTAERFGGQRAKEIG
LLSESYPSETLDEQVDRWIDNLLLNSPAAMRASKELLREVGNGALTPALRRYTENAIARI
RVSPEGQEGLRAFLQKRAPNWQAEPNPTASKRKEPRQ