Protein Info for PFR28_01064 in Pseudomonas sp. RS175

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02963: xanthine dehydrogenase, small subunit
  • amino acids 2 to 470 (469 residues), 703.7 bits, E=5e-216
• PF00111: Fer2
  • amino acids 17 to 76 (60 residues), 27.9 bits, E=3.6e-10
• PF01799: Fer2_2
  • amino acids 87 to 158 (72 residues), 97.6 bits, E=7.3e-32
• PF00941: FAD_binding_5
  • amino acids 199 to 362 (164 residues), 175.5 bits, E=1.7e-55
• PF03450: CO_deh_flav_C
  • amino acids 370 to 470 (101 residues), 126.7 bits, E=7.3e-41

Best Hits

KEGG orthology group: K13481, xanthine dehydrogenase small subunit [EC: 1.17.1.4] (inferred from 93% identity to pba:PSEBR_a1703)

Predicted SEED Role

"Xanthine dehydrogenase, iron-sulfur cluster and FAD-binding subunit A (1.17.1.4)" in subsystem Purine Utilization

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• superpathway of purines degradation in plants (14/18 steps found)
• ureide biosynthesis (6/7 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4

Protein Sequence (484 amino acids)

>PFR28_01064 hypothetical protein (Pseudomonas sp. RS175)
MIQFLLNQELRSEHSLDPNLTVLNYLREHVGRSGTKEGCASGDCGACTVVVGELHVDESG
HERLRYRSLNSCLTFVSSLHGKQLISVEDLKHQGRLHSVQQAMVDCHGSQCGFCTPGFVM
SLFALQKNSEQPDAHKAHEALAGNLCRCTGYRPILAAADQACCGKQTDQFDAREAQTIAR
LKAIAPKETGELNSGDKRCLVPLTVADLAELYDAYPQARLLAGGTDLALEVTQFHRTLPV
MIYVGNVAELKRIERFDDRLEIGAATALSDCYEALQAEYPDFGELLQRFASLQIRNQGTL
GGNIGNASPIGDSPPLLIALGAQIVLCKGLVRRTLALEDYFIDYRVTARQESEFIEKIIV
PRASAEQAFRAYKVSKRLDDDISAVCAAFNLRIDNGVVRDARVAFGGMAATPKRAKHCEA
ALIGSAWNDSTVERACAALGEDFTPLSDFRASKEYRLLGARNLLRKYFIELQTPHIETRV
TAYV