Protein Info for PFR28_01040 in Pseudomonas sp. RS175

Annotation: Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 163 TIGR01935: RraA family" amino acids 6 to 155 (150 residues), 241.7 bits, see alignment E=1.2e-76 PF03737: RraA-like" amino acids 6 to 154 (149 residues), 163.6 bits, see alignment E=2e-52

Best Hits

Swiss-Prot: 98% identical to RRAAH_PSEF5: Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (PFL_1871) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K02553, regulator of ribonuclease activity A (inferred from 98% identity to pfl:PFL_1871)

Predicted SEED Role

"Ribonuclease E inhibitor RraA" in subsystem RNA processing and degradation, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (163 amino acids)

>PFR28_01040 Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (Pseudomonas sp. RS175)
MKHYLTPDLCDAYPDLVQVVEPMFSNFGGRDSFGGEIVTIKCFEDNSLVKEQVELKGNGK
VLVVDGGGSLRRALLGDMLAEKAAKNGWEGLVIYGCIRDVDVIAQTDLGVQALASHPMKT
DKRGIGDLNVAVTFAGVTFRPGEYVYADNNGVIVSPSPLQMPE