Protein Info for PFR28_00940 in Pseudomonas sp. RS175

Annotation: Potassium-transporting ATPase ATP-binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 685 transmembrane" amino acids 45 to 65 (21 residues), see Phobius details amino acids 71 to 92 (22 residues), see Phobius details amino acids 224 to 247 (24 residues), see Phobius details amino acids 257 to 281 (25 residues), see Phobius details amino acids 590 to 609 (20 residues), see Phobius details amino acids 619 to 638 (20 residues), see Phobius details amino acids 658 to 681 (24 residues), see Phobius details TIGR01497: K+-transporting ATPase, B subunit" amino acids 22 to 680 (659 residues), 961.1 bits, see alignment E=2.7e-293 TIGR01494: HAD ATPase, P-type, family IC" amino acids 80 to 348 (269 residues), 106.9 bits, see alignment E=9.2e-35 amino acids 372 to 605 (234 residues), 152.4 bits, see alignment E=1.5e-48 PF00122: E1-E2_ATPase" amino acids 118 to 289 (172 residues), 100.6 bits, see alignment E=1.6e-32 PF00702: Hydrolase" amino acids 308 to 533 (226 residues), 86.7 bits, see alignment E=5.6e-28 PF12710: HAD" amino acids 445 to 529 (85 residues), 28.8 bits, see alignment E=3.3e-10

Best Hits

Swiss-Prot: 86% identical to KDPB_PSEAE: Potassium-transporting ATPase ATP-binding subunit (kdpB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01547, K+-transporting ATPase ATPase B chain [EC: 3.6.3.12] (inferred from 93% identity to pba:PSEBR_a1587)

Predicted SEED Role

"Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)" in subsystem Potassium homeostasis (EC 3.6.3.12, TC 3.A.3.7.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.12

Use Curated BLAST to search for 3.6.3.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (685 amino acids)

>PFR28_00940 Potassium-transporting ATPase ATP-binding subunit (Pseudomonas sp. RS175)
MMNMPATETVATKATQSPKTSVATLWRPALKQAFVKLDPRQLYRAPVMLVVELTAVFTTV
LCFIPDSVVPVFVAAQIALWLWFTVLFANFAEALAEGRGKARADSLKAGSEGLSARRQVA
AGFEVIPASGLRKGDVVRVEAGEMIPGDGEVIEGIAAVNEAAITGESAPVIRESGGDRSA
VTGNTRLVSDWLLVRITSNPGESTLDRMIALVEGAKRQKTPNEVALDILLIGLTLIFLLV
VVTLQPFAHFANGSLPLVFLVALLVTLIPTTIGGLLSAIGIAGMDRLVRLNVIAKSGRAV
EAAGDVHVLMLDKTGTITFGNRRCAAVYATPGVSAKELAEGALFASLADETAEGKSIVEY
LRGLHPQPEPAVELLTAVPFSAETRLSGVDYQGRKYRKGAVDSLLEFVGLPRSELPTVLA
REIDKIAQSGGTPLLVCVDGRLLGAIHLKDVVKPGIRERFAELRKLGIRTVMVTGDNPLT
AAAIAAEAGVDDVLAEATPEKKLARIRHEQNDGRLVAMCGDGANDAPALAQADVGMAMND
GTQAAREAANMVDLDSDPTKLLDVVQIGKELLVTRGALTTFSIANDIAKYFAILPALFAS
IYPQLGVLNVMHLNSPQSAIVSAIVFNALIIVVLIPLALRGVRVQAASAAALLRRNLLIY
GLGGIVVPFVGIKAIDMLLTALNLV