Protein Info for PFR28_00855 in Pseudomonas sp. RS175

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 760 transmembrane" amino acids 619 to 637 (19 residues), see Phobius details PF01627: Hpt" amino acids 8 to 100 (93 residues), 64.8 bits, see alignment E=1.7e-21 PF02895: H-kinase_dim" amino acids 374 to 433 (60 residues), 56.7 bits, see alignment 6.9e-19 PF02518: HATPase_c" amino acids 481 to 620 (140 residues), 64.3 bits, see alignment E=3.4e-21 PF01584: CheW" amino acids 627 to 754 (128 residues), 94 bits, see alignment E=1.6e-30

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 89% identity to pba:PSEBR_a1501)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (760 amino acids)

>PFR28_00855 hypothetical protein (Pseudomonas sp. RS175)
MSFGADEEILQDFLVEAGEILEQLSEQLVELESRPDDADLLNAIFRGFHTVKGGAGFLQL
NELVECCHIAENVFDILRKGERRVDSELMDVVLEALDAVNSMFSEVRERSPITPATPELL
AALSRLAEPQSADEAAPAEEPAVEEPVAEEPVAQESGDITDNEFEQLLDSLSAVKAEAQV
QAPVAPAAQAADGTASDEITDAEFESLLDQLHGKGQFAPDAVAPTPTPAAPSAAGDNSDI
TDDEFEALLDQLHGKGTFAVDALESAIASAPGSDKPSAAAAGSDLITDQEFESLLDELHG
KGKFSEVGTATAASTAAPAAKAAPKPAAKAPESKAPQAKAETPAPAVAAKPAAPAPARAA
AAAPAEKPTSEAETTVRVDTARLDEIMNMVGELVLVRNRLVRLGLNSQDEAMSKAVSNLD
VVTADLQTAVMKTRMQPIKKVFGRFPRLVRDLARQLKKEINLELVGEETDLDKNLVEALA
DPLVHLVRNAVDHGIESPEEREAAGKVRSGKVILAAEQEGDHILLSISDDGKGMDPEILR
AIAVKRGVMDKDAADRLSDTECYNLIFAPGFSTKTEISDVSGRGVGMDVVKTKISQLNGS
INIHSVKGSGSKILIKVPLTLAIMPTLMVMLGNQAFAFPLVNVNEIFHLDLSKTNVVDGQ
EVVIVRDKALPLFYLKRWLVSSAAHVEQGEGHVVILSVGTQRIGFVVDQLVGQEEVVIKP
LGKMLQGTPGMSGATITGDGRIALILDVPSMLKRYATRRI