Protein Info for PFR28_00756 in Pseudomonas sp. RS175

Annotation: ATP-dependent RNA helicase RhlE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 PF00270: DEAD" amino acids 24 to 187 (164 residues), 155.2 bits, see alignment E=2.1e-49 PF04851: ResIII" amino acids 37 to 185 (149 residues), 34 bits, see alignment E=4.1e-12 PF00271: Helicase_C" amino acids 232 to 338 (107 residues), 101.1 bits, see alignment E=6.8e-33

Best Hits

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a1411)

Predicted SEED Role

"ATP-dependent RNA helicase SrmB" in subsystem ATP-dependent RNA helicases, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>PFR28_00756 ATP-dependent RNA helicase RhlE (Pseudomonas sp. RS175)
MFSQFALHERLLKAVAELKFVEPTPVQAAAIPLALQGRDLRVTAQTGSGKTAAFVLPILN
RLIGPAKVRVSIKTLILLPTRELAQQTLKEVERFSQFTFIKSGLITGGEDFKVQAAMLRK
VPDILIGTPGRMIEHLNAGNLDLKEVEVLVLDEADRMLDMGFAEDVQRLVDECSQRQQTM
LFSATTGGSGLREMVAKVLNNPEHLQLNAVSQLNSTTRQQIITADHNQHKEQIVNWLLAN
ETYEKAIVFTNTRAMADRIYGRLVAQEYKAFVLHGEKDQKDRKLAIDRLKQGGVKILVAT
DVAARGLDVEGLDMVINFDMPRSGDEYVHRIGRTGRAGNDGLAISLICHGDWNLMSSIER
YLKQSFERRTIKEVKGTYSGPKKVKASGKAVGVKKKKVDAKGDKKKAGAKAPTKRKTANR
PKTEAPALVSKDGMAPLKRRKPQAPAAE