Protein Info for PFR28_00566 in Pseudomonas sp. RS175

Annotation: putative sugar-binding periplasmic protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details

Best Hits

Swiss-Prot: 47% identical to SP39_RHIME: Probable sugar-binding periplasmic protein (R03301) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02027, multiple sugar transport system substrate-binding protein (inferred from 97% identity to pba:PSEBR_a1213)

Predicted SEED Role

"Glucose ABC transport system, periplasmic sugar-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (433 amino acids)

>PFR28_00566 putative sugar-binding periplasmic protein (Pseudomonas sp. RS175)
MNAISRLATVISLASLLPVAAFPVSTLAAESKGSVEVVHWWTSGGEKAAVDVLKAQVEKD
GFTWKDGAVAGGGGSTAMTVLKSRAVAGNPPGVAQIKGPDIQEWATTGLLDTDVLKDVAK
QEKWDSLLDKKVSDTVKYDGDYVAVPVNIHRVNWLWINPEVFKKAGITKNPTTLEEFYAA
GDKLKAAGFIPLAHGGQPWQDSTVFEAVVLSVMGADGYKKALVDLDNKALTGPEMIKALT
ELKKVATYMDADGKGQDWNLEAAKVINGKAGMQIMGDWAKSEWTAAKKVAGKDYECVAFP
GTDKAFTYNIDSLAVFKQKDEGTAAGQQDIAKVVLGENFQKVFSINKGSIPVRNDMLADM
AKYGFDSCAQTAAKDFLADAKNGGLQPSMAHNMATTLAVQGAFFDVVTNYINDPKADPAD
AAKKLGAAVQSAK