Protein Info for PFR28_00545 in Pseudomonas sp. RS175

Annotation: p-hydroxybenzoate hydroxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02360: 4-hydroxybenzoate 3-monooxygenase
  • amino acids 4 to 392 (389 residues), 682.4 bits, E=8.5e-210
• PF01494: FAD_binding_3
  • amino acids 5 to 346 (342 residues), 341.1 bits, E=8.6e-106

Best Hits

Swiss-Prot: 76% identical to PHHY_PSEFL: p-hydroxybenzoate hydroxylase (pobA) from Pseudomonas fluorescens

KEGG orthology group: K00481, p-hydroxybenzoate 3-monooxygenase [EC: 1.14.13.2] (inferred from 92% identity to pba:PSEBR_a1192)

MetaCyc: 76% identical to p-hydroxybenzoate hydroxylase (Pseudomonas fluorescens)
4-hydroxybenzoate 3-monooxygenase. [EC: 1.14.13.2]

Predicted SEED Role

"P-hydroxybenzoate hydroxylase (EC 1.14.13.2)" in subsystem p-Hydroxybenzoate degradation (EC 1.14.13.2)

MetaCyc Pathways

• 4-chlorobenzoate degradation (2/4 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• 4-coumarate degradation (aerobic) (2/5 steps found)
• 4-methylphenol degradation to protocatechuate (1/4 steps found)
• 4-hydroxymandelate degradation (2/6 steps found)
• bisphenol A degradation (1/5 steps found)
• spongiadioxin C biosynthesis (2/7 steps found)
• polybrominated dihydroxylated diphenyl ethers biosynthesis (2/8 steps found)
• superpathway of aerobic toluene degradation (11/30 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (13/35 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (10/42 steps found)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation
• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.2

Use Curated BLAST to search for 1.14.13.2

Protein Sequence (396 amino acids)

>PFR28_00545 p-hydroxybenzoate hydroxylase (Pseudomonas sp. RS175)
MKTLKTQVAIIGAGPSGLLLGQLLHNAGIDTLILERQTPDYVLGRIRAGVLEQGMAELLR
QAGVGQRMDAEGLPHDGFELALNGRRVHIDLKGLTGGKNVMVYGQTEVTRDLMAARQSSG
GRTLYEVEKAQPHDMQTEHPFVTFEHQGQAWRLDCDYIAGCDGFHGVARQSIPAENLKVF
ERVYPFGWLGILADTPPVHEELVYARHERGFALCSMRSRTRTRYYLQVPAQEQVADWPDE
RFWAELKRRLPADLAEALVTGPSIEKSIAPLRSFVVEPMQYGRMFLVGDAAHIVPPTGAK
GLNLAASDVSTLFNILLKVYREGRVDLLQQYSAICLRRVWKAERFSWWMTSMLHRFDDDA
FDQRISQAELEYFVDSEAGRKTIAENYVGLPYEAIE