Protein Info for PFR28_00480 in Pseudomonas sp. RS175

Annotation: HTH-type transcriptional activator CmpR

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 transmembrane" amino acids 116 to 139 (24 residues), see Phobius details PF00126: HTH_1" amino acids 30 to 88 (59 residues), 64.6 bits, see alignment E=9.3e-22 PF03466: LysR_substrate" amino acids 116 to 316 (201 residues), 195.9 bits, see alignment E=8.3e-62 PF12727: PBP_like" amino acids 185 to 314 (130 residues), 29.8 bits, see alignment E=4.8e-11

Best Hits

Swiss-Prot: 44% identical to YEIE_ECO57: Uncharacterized HTH-type transcriptional regulator YeiE (yeiE) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a1130)

Predicted SEED Role

"LysR family transcriptional regulator PA3398" in subsystem DNA-binding regulatory proteins, strays

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (333 amino acids)

>PFR28_00480 HTH-type transcriptional activator CmpR (Pseudomonas sp. RS175)
MHRILIAATISVKWIIKIRVIGYIDMRFTLRQLQVFVAVAQQESVSRAAGQLNLSQSAAS
TSITELERQSSCQLFDRAGKRLSLNALGKQLLPQAVALLDQAKEIEDLLNGKSGFGSLAV
GATLTIGNYLATLLIGGFMQRHPESQVKLHVQNTANIVHQVAHYEIDLGLIEGDCSHPDI
EVQSWVEDELVVFCAPQHPLARRGQATMEELTHEAWILREQGSGTRLTFDQAMRHHRSAL
NIRLELEHTEAIKRAVESGLGIGCISRLALRDAFRRGSLVPVETPDMDLARQFYFIWHKQ
KYQTSAMREFLELCRAFTAGAQRSDEIVLPAIA