Protein Info for PFR28_00427 in Pseudomonas sp. RS175

Annotation: RNA polymerase sigma factor RpoS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 336 TIGR02394: RNA polymerase sigma factor RpoS" amino acids 58 to 335 (278 residues), 458.3 bits, see alignment E=1.1e-141 PF00140: Sigma70_r1_2" amino acids 63 to 96 (34 residues), 43.9 bits, see alignment 3.8e-15 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 98 to 324 (227 residues), 117.8 bits, see alignment E=3.8e-38 PF04542: Sigma70_r2" amino acids 101 to 170 (70 residues), 82.4 bits, see alignment E=3.3e-27 PF04539: Sigma70_r3" amino acids 181 to 255 (75 residues), 74.2 bits, see alignment E=1.4e-24 PF04545: Sigma70_r4" amino acids 269 to 322 (54 residues), 51.3 bits, see alignment 1.3e-17

Best Hits

Swiss-Prot: 89% identical to RPOS_PSEAE: RNA polymerase sigma factor RpoS (rpoS) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03087, RNA polymerase nonessential primary-like sigma factor (inferred from 98% identity to pba:PSEBR_a1096)

MetaCyc: 76% identical to RNA polymerase sigma factor RpoS (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"RNA polymerase sigma factor RpoS" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (336 amino acids)

>PFR28_00427 RNA polymerase sigma factor RpoS (Pseudomonas sp. RS175)
MALSKEVPEFDIDDEVLLMETGIAMDSMSNDEGASSPPSVRAKSRHSASLKQHKYIDYTR
ALDATQLYLNEIGFSPLLSPEEEVHFARLSQSGDPAGRKRMIESNLRLVVKIARRYVNRG
LSLLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVK
ELNVYLRAARELTQKLDHEPSPEEIANLLEKPVGEVKRMLGLNERVSSVDVSLGPDSDKT
LLDTLTDERPTDPCELLQDDDLSQSIDQWLSELTDKQREVVIRRFGLRGHESSTLEDVGL
EIGLTRERVRQIQVEGLKRLREILEKNGLSSESLFQ