Protein Info for PFR28_00421 in Pseudomonas sp. RS175

Annotation: S-formylglutathione hydrolase YeiG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 5 to 279 (275 residues), 431.1 bits, E=1e-133
• PF00756: Esterase
  • amino acids 23 to 273 (251 residues), 232 bits, E=9.6e-73
• PF00326: Peptidase_S9
  • amino acids 130 to 262 (133 residues), 26.7 bits, E=3.6e-10

Best Hits

Swiss-Prot: 62% identical to SFGH2_ECO57: S-formylglutathione hydrolase YeiG (yeiG) from Escherichia coli O157:H7

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 96% identity to pba:PSEBR_a1090)

MetaCyc: 61% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of methylglyoxal degradation (3/8 steps found)
• superpathway of C1 compounds oxidation to CO₂ (5/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Protein Sequence (284 amino acids)

>PFR28_00421 S-formylglutathione hydrolase YeiG (Pseudomonas sp. RS175)
MNLENISCQKSFGGWHKRYRHRSEVLGCDMVFAVYLPPQAEQGGKLPVLYWLSGLTCTDE
NFMQKAGAQRMAAELGLIIVAPDTSPRGADVPGDPDGAWDFGLGAGFYLNATQEPWARHY
RMYDYVVQELPALVETHFPASDKRSISGHSMGGHGALVCALRNPGRYQSVSAFSPITNPM
DCPWGQKAFSRYLGEDRSKWREWDACALIAEAEEKLPLLVDQGDRDDFLANQLKPEALQQ
AAKAAGHPLELRLQPGYDHSYYFIASFIDDHLQHHARALGRAIG