Protein Info for PFR28_00419 in Pseudomonas sp. RS175

Annotation: HTH-type transcriptional regulator DmlR

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 298 PF00126: HTH_1" amino acids 12 to 65 (54 residues), 75.2 bits, see alignment E=3.2e-25 PF03466: LysR_substrate" amino acids 90 to 290 (201 residues), 164.3 bits, see alignment E=2.6e-52

Best Hits

Swiss-Prot: 33% identical to Y1364_HAEIN: Uncharacterized HTH-type transcriptional regulator HI_1364 (HI_1364) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a1088)

Predicted SEED Role

"Transcriptional regulator, LysR family, in formaldehyde detoxification operon" in subsystem Glutathione-dependent pathway of formaldehyde detoxification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (298 amino acids)

>PFR28_00419 HTH-type transcriptional regulator DmlR (Pseudomonas sp. RS175)
MSDNRWEGIDEFVAVAECSQFTAAAERLGVSSSHISRQIVRLEERLQTRLLYRSTRRVTL
TEAGQTFLQHCQRLQDGREEALRAVGDLASEPKGMLRMTCAVAYGERFIVPLVTRFMGLY
PQLRVDIELSNRPLDLVHEGLDLAIRLGRLQDSRLIATRLAPRRMYLCASPSYLERYGRP
HSLSELSRHNCLIGSSDLWQLEQNGREFSQRVQGNWRCNSGQAVLDAALQGVGLCQLPDY
YVLEHLHNGALISLLEAHQPPNTAVWALYPQQRHLSPKVRKLVDYLKEGLAQRPEYRG