Protein Info for PFR28_00257 in Pseudomonas sp. RS175

Annotation: C4-dicarboxylate-binding periplasmic protein DctP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 331 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF03480: DctP" amino acids 31 to 314 (284 residues), 353.3 bits, see alignment E=5.2e-110 TIGR00787: TRAP transporter solute receptor, DctP family" amino acids 31 to 276 (246 residues), 254.7 bits, see alignment E=4.7e-80

Best Hits

Swiss-Prot: 69% identical to DCTP_PSEAE: C4-dicarboxylate-binding periplasmic protein DctP (dctP) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 88% identity to pba:PSEBR_a874)

Predicted SEED Role

"TRAP-type C4-dicarboxylate transport system, periplasmic component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (331 amino acids)

>PFR28_00257 C4-dicarboxylate-binding periplasmic protein DctP (Pseudomonas sp. RS175)
MLNVFWKTLVFGLAVGLLGTAMAADPIVIKFSHVVAEQTPKGQGALMFKKLVEERLPGRV
KVEVYPNSTLYGDDKEMEALLLGEVQIIARSLAKFEQYTKSVQLFDLPFLFDDIAAVDRF
QQSPEGQKLLKSMEKKNVTGLAYWHNGMKQLSANRPLRVPEDARGLTFRIQTSAVLDEQF
KAVGAKAQPMIFSVVYQGLRTGLVNGTENTYSNFYNQKLNEVQKYVTESNHGILDYMLIT
TSDFWNGLPPDIRSELDRIVVESTAHANKEAERFNQQDRQQVLDAKTTEIITLTPQERSA
WRDRMKPVWTKFEKEIGADLIKAAEASNTTQ